AB324630

Plasmin Activity Assay Kit (Fluorometric)

Name: Plasmin Activity Assay Kit (Fluorometric) (ab324630) | Abcam
Brand: Abcam Limited
SKU: AB324630

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Plasmin Activity Assay Kit (Fluorometric) is based on the ability of Plasmin to proteolytically cleave a synthetic plasmin substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence microplate readers at Ex/Em = 360/450 nm. This assay kit is simple, rapid and can detect plasmin activity as low as 8.66 µU in a variety of samples.

別名を表示する

Plasminogen

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Functional Studies - Plasmin Activity Assay Kit (Fluorometric) (AB324630)

FuncS

Supplier Data

Functional Studies - Plasmin Activity Assay Kit (Fluorometric) (AB324630)

Quantification of plasmin activity in donor-pooled human plasma samples (5 µl/well). Plasmin activity was measured in activated human plasma samples in the presence or absence of the plasmin inhibitor, Aprotinin. P = Unactivated plasma (plasmin typically circulates as the inactive zymogen plasminogen), AB = Plasma preincubated with activation buffer containing urokinase (which converts the inactive zymogen plasminogen to the active enzyme, plasmin) for 10 minutes at 37°C. Assay was performed following the kit protocol.

FuncS

Supplier Data

Functional Studies - Plasmin Activity Assay Kit (Fluorometric) (AB324630)

Standard curve of 7-amino-4-methylcoumarin (AMC) fluorescence, measured at Ex/Em = 368/460 nm. One mole of AMC corresponds to the metabolism of one mole of Plasmin Substrate.

Key facts

検出方法

Fluorometric

サンプルタイプ

EDTA Plasma

交差種

Human

アッセイタイプ

Enzyme activity

Quantitative

検出感度

= 8.66 µU

アッセイ時間

アッセイプラットフォーム

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Plasmin (EC 3.4.21.7) is a serine protease occurring in plasma as plasminogen. Upon activation via cleavage by plasminogen activators; plasmin solubilizes fibrin clots and activates and/or degrades compounds of the coagulation and complement systems. Plasminogen-Plasmin system has also been implicated in a wide variety of physiologic and pathologic processes, including tumor growth, invasion and metastasis.

Technical Note: Normal plasma has very low constitutive activity. Plasma must be activated by pretreatment with urokinase or streptokinase.

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精度

[ { "reproducibilityType": "Intra", "sample": "EDTA Plasma", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.9" }, { "reproducibilityType": "Inter", "sample": "EDTA Plasma", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "0.079" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab324630-1x96Tests", "assetComponentDetails": [ { "size":"1 x 400 µL", "name":"Plasmin Substrate", "number":"AB324630-CMP04", "productcode":"" }, { "size":"1 x 15 mL", "name":"Plasmin Assay Buffer", "number":"AB324630-CMP02", "productcode":"" }, { "size":"1 x 1.5 mL", "name":"Plasmin Dilution Buffer", "number":"AB324630-CMP01", "productcode":"" }, { "size":"1 x 100 µL", "name":"AMC Standard (1 mM AMC in DMSO)", "number":"AB324630-CMP05", "productcode":"" }, { "size":"1 x 15 µL", "name":"Plasmin Positive Control", "number":"AB324630-CMP03", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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製品プロトコール

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ターゲットの情報

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1, C4 and C5 (PubMed : 6447255). Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells.. Angiostatin is an angiogenesis inhibitor that blocks neovascularization and growth of experimental primary and metastatic tumors in vivo.. (Microbial infection) ENO/enoloase from parasite P.falciparum (strain NF54) interacts with PLG present in the mosquito blood meal to promote the invasion of the mosquito midgut by the parasite ookinete (PubMed : 21949403). The catalytic active form, plasmin, is essential for the invasion of the mosquito midgut (PubMed : 21949403).. (Microbial infection) Binds to OspC on the surface of B.burgdorferi cells, possibly conferring an extracellular protease activity on the bacteria that allows it to traverse host tissue.. (Microbial infection) Interacts with dengue virus type 2 particles (PubMed : 31726374). Enhances dengue virus type 2 infection in Aedes aegypti mosquito midgut by increasing midgut internalization, resulting in higher infection rates and viral dissemination in mosquitoes (PubMed : 31726374).

See full target information Plasmin

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websiteProtocolBooklet

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Plasmin Activity Assay Kit (Fluorometric)