Picro Sirius Red Stain Kit (Connective Tissue Stain) (ab150681)
Key features and details
- Assay time: 1 hr
- Sample type: Tissue sections
製品の概要
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製品名
Picro Sirius Red Stain Kit (Connective Tissue Stain) -
サンプルの種類
Tissue sections -
全工程の試験時間
1h 00m -
製品の概要
Picro sirius red staining has traditionally sometimes been used with polarized light to more easily differentiate the collagen fibres from the background. Studies have used the differences between the appearance of different collagen fibres under polarized light to differentiate between different types of collagen fibres; it is unclear if the differences in appearance of the fibres is due to orientation, thickness and packing of the fibres rather than reflecting different types of collagen fibers.
Picro sirius red staining protocol summary
- Deparaffinize sections if necessary and hydrate in distilled water
- Cover sections in picro-sirius red solution and incubate for 60 min
- Wash slide with acetic acid solution
- Wash slide with absolute alcohol
- Dehydrate, clear and mount slide
Getting the best performance from ab150681
ab150681 may not be suitable for frozen sections.
Related and recommended products
See below other connective tissue stains to highlight and identify different types of connective tissue in tissue samples with different dyes:
- Trichrome Stain Kit (Connective Tissue Stain) ab150686
- Phosphotungstic Acid Hematoxylin (PTAH) Stain Kit (Connective Tissue Stain) ab150683
- Reticulum Stain Kit (Connective Tissue Stain) ab150684 -
特記事項
Staining Interpretation
Light Microscopy
Collagen Red Muscle Fibers Yellow Cytoplasm Yellow Polarized Light Microscopy
Type I (Thick fibers) Yellow-Orange Birefringence Type III (Thin fibers) Green Birefringence Control Tissue: Lung, Uterus, Muscle, Kidney.
製品の特性
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保存方法
Store at room temperature. Please refer to protocols. -
内容 100 tests Acetic Acid Solution 2 x 250ml Picro Sirius Red Solution 1 x 250ml -
研究分野
画像
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ab150681 - Picro Sirius Red Stain Kit (Connective Tissue Stain)
ab150681 Picro Sirius Red Stain Kit (Connective Tissue Stain) staining formalin-fixed-paraffin embedded human skeletal muscle.
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ab150681 - Picro Sirius Red Stain Kit (Connective Tissue Stain)ab150681 (Picro Sirius Red stain) staining collagen (red), muscle fibres (yellow), cytoplasm (yellow) and red blood cells (yellow) in formalin fixed paraffin embedded normal human colon.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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ab150681 - Picro Sirius Red Stain Kit (Connective Tissue Stain)Kumar P et al. PLoS One (2014) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/ab150681 staining mouse liver sections in control (saline group) showing normal lobular structure; in CCl4 treated showing extensive collagen deposition and bridging fibrosis; and in CCl4-AND treated mice which showed decreased collagen deposition.
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Evaluation of liver tissues fibrosis pattern using Picro Sirius Red Stain Kit (ab150681)This image is taken from Deodati A. et al. PLoS One. 2018; 13(6): e0198490. Fig 3e. doi: 10.1371/journal.pone.0198490. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.Evaluation of liver tissues fibrosis pattern in IUGR and SHAM adult males rats, using Picro-Sirius Red Stain (ab150681). Values are expressed as fold change (mean + SD, * p<0.01).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (324)
ab150681 は 324 報の論文で使用されています。
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- Li J et al. A Mammalian Conserved Circular RNA CircLARP1B Regulates Hepatocellular Carcinoma Metastasis and Lipid Metabolism. Adv Sci (Weinh) 11:e2305902 (2024). PubMed: 37953462
- Reid VJM et al. Assessment of the alpha 7 nicotinic acetylcholine receptor as an imaging marker of cardiac repair-associated processes using NS14490. EJNMMI Res 14:7 (2024). PubMed: 38206500
- Uddin MH et al. Molecular analysis of XPO1 inhibitor and gemcitabine-nab-paclitaxel combination in KPC pancreatic cancer mouse model. Clin Transl Med 13:e1513 (2023). PubMed: 38131168
- Park J et al. Scaffold-induced compression enhances ligamentization potential of decellularized tendon graft reseeded with ACL-derived cells. iScience 26:108521 (2023). PubMed: 38162024