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Phenylalanine Assay Kit (ab83376)

Submit a review Q&A (4)References (1)
OEM testing Phase VI ; Kits & Assays Initiative Assay Testing Jan/Feb 2015
  • Functional assay - ab83376 Phenylalanine Assay Kit
  • Functional assay - ab83376 Phenylalanine Assay Kit

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Assay time: 1 hr
  • Sample type: Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
  • Sensitivity: 2 µM

製品の概要

  • 製品名

    Phenylalanine Assay Kit

  • 検出方法

    Fluorescent

  • サンプルの種類

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts

  • アッセイタイプ

    Quantitative

  • 検出感度

    > 2 µM

  • 検出範囲

    2 µM - 20 µM

  • 全工程の試験時間

    1h 00m

  • 製品の概要

    Phenylalanine Assay Kit ab83376 provides a quick, simple, accurate method for quantifying phenylalanine in biological samples.


    In the phenylalanine assay protocol, phenylalanine is reductively deaminated with the simultaneous formation of NADH which reacts with our fluorescent probe to generate fluorescence at Ex/Em=535/587 nm. The assay is linear in the range from 0.1 to 1.0 nmol (2-20 µM) of Phenylalanine.


    Phenylalanine assay protocol summary:
    - add samples and standards to wells
    - add reaction mix and incubate for 60 min
    - analyze with a microplate reader

  • 特記事項

    This product is manufactured by BioVision, an Abcam company and was previously called K572 Phenylalanine Fluorometric Assay Kit. K572-100 is the same size as the 100 test size of ab83376.

    L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin.

    The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.

  • 試験プラットフォーム

    Microplate reader

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 100 tests
    Phenyalanine Standard 1 vial
    Fructose Assay Buffer 1 x 25ml
    Phenylalanine Developer Mix 1 vial
    Phenylalanine Enzyme Mix 1 vial
    Tyrosinase 1 vial

  • 研究分野

  • 関連性

    L-Phenylalanine (PHE) is an electrically-neutral amino acid, one of the twenty common and one of the three aromatic amino acids used to biochemically form proteins. Phenylalanine uses the same active transport channel as tryptophan to cross the blood-brain barrier, and, in large quantities, interferes with the production of serotonin. Errors in PHE metabolism lead to phenylketonuria or PKU which can have dire consequences.

  • 別名

    • Phe

関連製品

画像

  • OEM testing Phase VI ; Kits & Assays Initiative Assay Testing Jan/Feb 2015
    OEM testing Phase VI ; Kits & Assays Initiative Assay Testing Jan/Feb 2015

    Phenylalanine measured in cell lysates showing quantities (micromoles) per million of cells tested.

  • Functional assay - ab83376 Phenylalanine Assay Kit
    Functional assay - ab83376 Phenylalanine Assay Kit

    Phenylalanine measured in biofluids showing concentration in micromolar.

  • Functional assay - ab83376 Phenylalanine Assay Kit
    Functional assay - ab83376 Phenylalanine Assay Kit

    Phenylalanine fluorimetric standard curve: mean of duplicates (+/- SD) with background readings subtracted

参考文献 (1)

ab83376 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab83376 は 1 報の論文で使用されています。

  • Carney BC  et al. Laser-assisted drug delivery of synthetic alpha melanocyte stimulating hormone and L-tyrosine leads to increased pigmentation area and expression of melanogenesis genes in a porcine hypertrophic scar model. Lasers Surg Med 55:490-502 (2023). PubMed: 37051852

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Question

What is the function of Tyrosinase vial provided in the kit?

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Answer

Thank you for contacting us.
The enzyme used in the assay can react with tyrosine and methionine as well as
phenylalanine. Serum methionine concentrations are generally low enough to be
insignificant in this assay. Tyrosine concentrations may interfere. If tyrosine interference is a concern, add 5 μl tyrosinase to the samples and preincubate for 10 minutes before
performing the assay to remove tyrosine interference.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Hello,



We were planning to analyse mouse tissue samples (brain, retina, lung)for phenylalanine content and would be interested to know if you have any further information about the amount of tissue that may be required for use with this kit (ab83376), as there are no specific reviews of the kit. The product datasheet recommends using20mg of tissue, haveyoureceived any feedback on use ofthis amount from other researchers and the readings obtained using the kit with such a sample? Also, are you aware of the kit being usedspecifically for mouse tissue?



Any additional information you can provide would be most helpful in determining if this is the correct kit for our needs.



Many thanks,

Read More
Answer

Thank youfor your inquiry.

Please excuse the delay in my answer. this kit was not tested in-house and I was in contact with the lab that tested it.

Unfortunately, actual data is proprietary information.

But I was assured that the kitis suitable for mouse samples.

We do not know of any publications using this kit at the moment, but will add them to the datasheet as soon as we know of any.

I would like to reassure you that ab83376 is guaranteed for mouse samples by our Abpromise guarantee:

https://www.abcam.com/abpromise

I am sorry that I could not provide all the answers on this occasion and hope this information is nevertheless helpful.

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Question

Was ist das Fluorochrom?

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Answer

Vielen Dank für Ihren Anruf.

Der im Phenylalanin-AssayKit ab83376verwendete Fluoreszenzfarbstoff (pico-probe) unterliegt zwar demBetriebsgeheimnis; allerdings wird er letztendlich in Resorufin umgewandelt - also genau, wie Sie schon vermutet hatten.

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Question

Well, I am working with an enzyme that is also a methylase that is involved in the N-methylation of a phenylalanine residue. I wonder if I could discriminate a phenylalanine that is methylated from other that is not with your kit?

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Answer

Thank you for contacting Abcam. We have not tested if this kit can differentiate between the two forms of phenyl alanine, that does not mean it cannot, it just means that we cannot guaranteed that it will. If there is anything else I can help you with, please let me know.

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