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NQO1 Activity Assay Kit (ab184867)

Submit a review Q&A (1)References (13)
Raw data from various concentrations of HepG2 cell extracts.
  • NQO1 Activity in HepG2 extract.
  • Raw data of NQO1 activity from 50 µg/mL of a series of normal samples.
  • Comparison of NQO1 activity in different samples at 50 ug/mL
  • Dicoumarol sensitive NQO1 activity/total activity in a series of normal cell lysates at 50 ug/mL.
  • NQO1 activity assay components requirement test.

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 30 min
  • Sample type: Adherent cells, Cell culture extracts, Cell Lysate, Suspension cells, Tissue Extracts, Tissue Homogenate, Tissue Lysate

製品の概要

  • 製品名

    NQO1 Activity Assay Kit

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Overall 3 2.183%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Overall 9 6.035%

  • サンプルの種類

    Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts, Cell Lysate, Tissue Homogenate, Tissue Lysate

  • アッセイタイプ

    Enzyme activity

  • 全工程の試験時間

    0h 30m

  • 種交差性

    交差種: Mouse, Rat, Cow, Human

  • 製品の概要

    NQO1 Activity Assay kit (ab184867) is designed for the sensitive and accurate measurement of NQO1 (NAD(P)H dehydrogenase [quinone] 1) activity.


    The enzyme activity is determined by following the reduction of Menadione with cofactor NADH and the simultaneous reduction of WST1 which leads to increased absorbance at 440 nm. Dicoumarol is used as an inhibitor of NQO1 as part of the assay.


    NQO1 assay protocol summary:
    - add samples to wells
    - add reaction buffer + inhibitor and reaction buffer to separate sample wells
    - analyze with a microplate reader for 5 min

  • 特記事項

    NQO1 serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways, NQO1 protects against quinone-induced damage by competing with potentially toxic one-electron pathways. It also functions in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

  • 試験プラットフォーム

    Microplate reader

製品の特性

画像

  • Raw data from various concentrations of HepG2 cell extracts.
    Raw data from various concentrations of HepG2 cell extracts.

    An example is shown below where the rate/slope is calculated between these time points.

  • NQO1 Activity in HepG2 extract.
    NQO1 Activity in HepG2 extract.

    This raw data is expressed as rate (mOD/min) per microgram of cell extract added per well as shown above. The molar extinction coefficient factor for the WST1 dye is 25.9/mM/well.

  • Raw data of NQO1 activity from 50 µg/mL of a series of normal samples.
    Raw data of NQO1 activity from 50 µg/mL of a series of normal samples.

    Figure 3a

  • Comparison of NQO1 activity in different samples at 50 ug/mL
    Comparison of NQO1 activity in different samples at 50 ug/mL

    Figure 3b

  • Dicoumarol sensitive NQO1 activity/total activity in a series of normal cell lysates at 50 ug/mL.
    Dicoumarol sensitive NQO1 activity/total activity in a series of normal cell lysates at 50 ug/mL.

    Figure 3c

  • NQO1 activity assay components requirement test.
    NQO1 activity assay components requirement test.

    Figure 4

参考文献 (13)

ab184867 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184867 は 13 報の論文で使用されています。

  • Iori S  et al. Deepening the Whole Transcriptomics of Bovine Liver Cells Exposed to AFB1: A Spotlight on Toll-like Receptor 2. Toxins (Basel) 14:N/A (2022). PubMed: 35878242
  • Akhtari FS  et al. High-throughput screening and genome-wide analyses of 44 anticancer drugs in the 1000 Genomes cell lines reveals an association of the NQO1 gene with the response of multiple anticancer drugs. PLoS Genet 17:e1009732 (2021). PubMed: 34437536
  • Pauletto M  et al. Discovering the Protective Effects of Resveratrol on Aflatoxin B1-Induced Toxicity: A Whole Transcriptomic Study in a Bovine Hepatocyte Cell Line. Antioxidants (Basel) 10:N/A (2021). PubMed: 34439473
  • Liu Y  et al. Fucoxanthin Pretreatment Ameliorates Visible Light-Induced Phagocytosis Disruption of RPE Cells under a Lipid-Rich Environment via the Nrf2 Pathway. Mar Drugs 20:N/A (2021). PubMed: 35049870
  • Farooqui Z  et al. Nrf2 inhibition induces oxidative stress, renal inflammation and hypertension in mice. Clin Exp Hypertens 43:175-180 (2021). PubMed: 33070655
View all Publications for this product

レビューと Q&A

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Question

Inquiry: Do you sell the HepG2 cell lysate which was used to measure enzyme activity, what is the catalogue number?

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Answer


I am sorry we do not cell HepG2 tissue lysate prepared using a suitable method for use with ab184867 NQO1 Activity Assay Kit. The lysates we sell are prepared for western blotting.

Sorry I am not able to provide a more positive answer on this occasion.

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