AB273334

Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric)

Name: Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric) (ab273334)
Brand: Abcam Limited
SKU: AB273334
Price: 48000 JPY
Availability: InStock
Rating: 5 (1 reviews)

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別名を表示する

Myeloperoxidase, MPO

3 Images

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Supplier Data

Functional Studies - Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric) (AB273334)

Example data.

Myeloperoxidase Activity (∆RFU +/- inhibitor) for freshly prepared white blood cell lysate (0.5μg), perfused rat spleen lysate (0.9μg), Positive Control, and reagent blank.

FuncS

Supplier Data

Functional Studies - Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric) (AB273334)

Example data.

Resorufin Standard Curve.

FuncS

Supplier Data

Functional Studies - Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric) (AB273334)

Example data.

Calculated Myeloperoxidase activity in white blood cell lysate and rat spleen lysate.

Key facts

検出方法

Fluorescent

サンプルタイプ

Tissue, Suspension cells, Adherent cells

アッセイタイプ

Enzyme activity

Quantitative

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric) (ab273334) oxidizes a substrate to generate fluorescence (Ex/Em = 535/587 nm), which is directly proportional to total peroxidase activity in the sample. A specific MPO inhibitor is provided, which suppresses peroxidase activity due to MPO. This permits differentiation of MPO-mediated peroxidation from the activity of other peroxidases which may be present in the samples.
This kit provides a sensitive, quick and easy method for detecting MPO activity and can detect less than 2 µU of MPO activity.

This product is manufactured by BioVision, an Abcam company and was previously called K747 Myeloperoxidase (MPO) Peroxidation Activity Assay Kit. K747-100 is the same size as the 100 test size of ab273334.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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製品内容

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab273334-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB273334-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"MPO Positive Control", "number":"AB273334-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Assay Buffer LVI", "number":"AB273334-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"MPO Inhibitor Control", "number":"AB273334-CMP03", "productcode":"" }, { "size":"1 x 50 µL", "name":"Hydrogen Peroxide Solution", "number":"AB273334-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"Resorufin Standard", "number":"AB273334-CMP06", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

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製品プロトコール

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ターゲットの情報

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Myeloperoxidase (MPO) Peroxidation Activity Assay Kit (Fluorometric)