AB111749

Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric)

Name: Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (ab111749) | Abcam
Brand: Abcam Limited
SKU: AB111749
Price: 106000 JPY
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(18 Publications)

Myeloperoxidase (MPO) Activity Assay Kit ab111749 provides a rapid, simple and reliable fluorometric assay to study MPO activity.

別名を表示する

Myeloperoxidase, MPO

2 Images

FuncS

Supplier Data

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (AB111749)

Standard Curve using this kit protocol (Ex/Em = 485/525 nm)

FuncS

Supplier Data

Functional Studies - Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric) (AB111749)

a. Measurement of MPO activity in WBC lysate (0.1 μg) and MPO Positive Control (3 μL). b. MPO specific activity in WBC lysate.

Key facts

検出方法

Fluorescent

サンプルタイプ

Tissue Extracts, Cell culture supernatant

アッセイタイプ

Enzyme activity

検出感度

> 0.0005 mU/well

アッセイ時間

30m

アッセイプラットフォーム

Microplate reader

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

製品の詳細

Myeloperoxidase (MPO) Activity Assay Kit ab111749 provides a rapid, simple and reliable fluorometric assay to study MPO activity.

In the MPO assay protocol, MPO catalyzes the production of sodium hypochlorite (NaClO) from hydrogen peroxide (H₂O₂) and sodium chloride (NaCl). Subsequently, NaClO reacts stoichiometrically with the free radical sensor Aminophenyl fluorescein (APF) to generate fluorescein, whose fluorescence can be detected at Ex/Em = 485/525 nm.

This MPO assay kit can be used to detect MPO activity as low as 0.5 μU per well.

MPO assay protocol summary:
- add fluoroscein standard to wells and analyze with a microplate reader
- add samples to wells
- add reaction mix
- analyze every 2-3 mins with a microplate reader in kinetic mode for at least 30 mins

This product is manufactured by BioVision, an Abcam company and was previously called K745 Myeloperoxidase (MPO) Fluorometric Activity Assay Kit. K745-100 is the same size as the 100 test size of ab111749.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

製品内容

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab111749-100Test", "assetComponentDetails": [ { "size":"1 x 50 µL", "name":"Fluorescence Standard IV", "number":"AB111749-CMP02", "productcode":"" }, { "size":"1 x 50 µL", "name":"Hydrogen Peroxide Solution", "number":"AB111749-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"MPO Chlorination Probe", "number":"AB111749-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 54", "number":"AB111749-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"MPO Positive Control", "number":"AB111749-CMP03", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Myeloperoxidase also called MPO is an enzyme that plays a critical role in the body's immune response. This protein has a mass of approximately 150 kDa and exists as a dimer composed of heavy and light polypeptide chains. Myeloperoxidase is prominently expressed in neutrophils and monocytes which are types of white blood cells important for combating infections. The enzyme catalyzes the production of hypochlorous acid and other reactive substances by utilizing hydrogen peroxide and chloride ions. These reactive substances help in neutralizing pathogens during the immune response.

Biological function summary

The generation of reactive oxygen species by myeloperoxidase is essential for microbicidal activity. Myeloperoxidase functions as part of the antimicrobial system in the phagosome which is the intracellular compartment where pathogens are degraded. This enzyme works in conjunction with other components of the immune system such as NADPH oxidase. By generating hypochlorous acid MPO contributes to the oxidative burst a rapid release of reactive oxygen species during the response to pathogens.

Pathways

Myeloperoxidase integrates into the immune defense and inflammatory pathways. In particular it is associated with the neutrophil degranulation pathway where it releases its enzymatic contents to fight off microbes. MPO also interacts with proteins involved in oxidative stress processes such as superoxide dismutase which moderates levels of reactive oxygen species in cells. These interactions ensure balance in the immune response preventing excessive tissue damage during inflammation.

Dysregulated MPO activity can contribute to the development of diseases. For instance myeloperoxidase is linked with atherosclerosis a cardiovascular condition where inflammation and oxidative stress lead to plaque formation in the arteries. It also associates with vasculitis an autoimmune disorder causing inflammation of blood vessels. Both disorders can relate to the inflammatory pathways that involve MPO and proteins like C-reactive protein which serves as a marker of inflammation. Understanding MPO's role in these conditions is important for effective therapeutic interventions.

style

left-column

製品プロトコール

style

left-column

ターゲットの情報

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed : 9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed : 25698971).

See full target information MPO

style

left-column

文献 (18)

Recent publications for all applications. Explore the full list and refine your search

Toxics 11: PubMed36668787

2023

Can Maternal Exposure to Air Pollution Affect Post-Natal Liver Development?

Applications

Unspecified application

Species

Unspecified reactive species

Yong Song,Ling Chen,Ellen Bennett,Amanda J Wheeler,Katherine Southam,Seiha Yen,Fay Johnston,Graeme R Zosky

Stem cell research & therapy 13:479 PubMed36153571

2022

TNFAIP6 defines the MSC subpopulation with enhanced immune suppression activities.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyun Li,Lei Yang,Xian Chen,Xiangjuan Chen,Lianghui Diao,Yong Zeng,Jianyong Xu

Journal of healthcare engineering 2022:8450673 PubMed35399858

2022

Gu-Ben-Zhi-Ke-Zhong-Yao Alleviated PM2.5-Induced Lung Injury via HMGB1/NF-B Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxiao Hou,Hongchun Zhang,Meng Jiang,Yina Wu,Tao Li,Luhong Cong,Jun Duan

The Journal of biological chemistry 298:101847 PubMed35314195

2022

Capsaicin inhibits intestinal Cl secretion and promotes Na absorption by blocking TRPV4 channels in healthy and colitic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hanxing Wan,Xiong Ying Chen,Fenglian Zhang,Jun Chen,Fenglan Chu,Zachary M Sellers,Feng Xu,Hui Dong

mSphere 7:e0084721 PubMed35044805

2022

Nontypeable Haemophilus influenzae Redox Recycling of Protein Thiols Promotes Resistance to Oxidative Killing and Bacterial Survival in Biofilms in a Smoke-Related Infection Model.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin C Hunt,Xin Xu,Amit Gaggar,W Edward Swords

Nutrients 13: PubMed33920187

2021

Whole and Isolated Protein Fractions Differentially Affect Gastrointestinal Integrity Markers in C57Bl/6 Mice Fed Diets with a Moderate-Fat Content.

Applications

Unspecified application

Species

Unspecified reactive species

Tara R Price,Sangeetha A Baskaran,Kristin L Moncada,Yasushi Minamoto,Cory Klemashevich,Arul Jayuraman,Jan S Sucholdoski,Luis O Tedeschi,Jörg M Steiner,Suresh D Pillai,Rosemary L Walzem

American journal of physiology. Endocrinology and metabolism 320:E150-E159 PubMed33284091

2020

Myostatin deficiency not only prevents muscle wasting but also improves survival in septic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Masayuki Kobayashi,Shingo Kasamatsu,Shohei Shinozaki,Shingo Yasuhara,Masao Kaneki

Journal of innate immunity 13:83-93 PubMed33045713

2020

PolyADP-Ribosylation of NFATc3 and NF-κB Transcription Factors Modulate Macrophage Inflammatory Gene Expression in LPS-Induced Acute Lung Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yunjuan Nie,Teja Srinivas Nirujogi,Ravi Ranjan,Brenda F Reader,Sangwoon Chung,Megan N Ballinger,Joshua A Englert,John W Christman,Manjula Karpurapu

Frontiers in medicine 7:201 PubMed32582723

2020

Kidney Ischemia-Reperfusion Elicits Acute Liver Injury and Inflammatory Response.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Shang,Susara Madduma Hewage,Charith U B Wijerathne,Yaw L Siow,Cara K Isaak,Karmin O

Nature communications 10:1985 PubMed31064987

2019

Regeneration of severely damaged lungs using an interventional cross-circulation platform.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon A Guenthart,John D O'Neill,Jinho Kim,Dawn Queen,Scott Chicotka,Kenmond Fung,Michael Simpson,Rachel Donocoff,Michael Salna,Charles C Marboe,Katherine Cunningham,Susan P Halligan,Holly M Wobma,Ahmed E Hozain,Alexander Romanov,Gordana Vunjak-Novakovic,Matthew Bacchetta

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Myeloperoxidase (MPO) Activity Assay Kit (Fluorometric)