AB117129

Methylated DNA Quantification Kit (Fluorometric)

Name: Methylated DNA Quantification Kit (Fluorometric) (ab117129) | Abcam
Brand: Abcam Limited
SKU: AB117129
Price: 115000 JPY
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(3 Publications)

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC).

別名を表示する

5 Methycytosine, 5-Me Cytidine, 5-Me Cytosine, 5-Methyl-Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-MethylCytosine, 5-mC, 5-meC, methyl CpG

1 Images

Functional Studies - Methylated DNA Quantification Kit (Fluorometric) (AB117129)

FuncS

Supplier Data

Functional Studies - Methylated DNA Quantification Kit (Fluorometric) (AB117129)

Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117129. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117129.

Key facts

検出方法

Fluorescent

サンプルタイプ

Saliva, Urine, Plasma, Tissue, Suspension cells, Adherent cells

検出感度

>= 50 pg/well

アッセイ時間

アッセイプラットフォーム

Microplate reader

style

left-column

製品の詳細

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.

Abcam's Methylated DNA Quantification Kit (Fluorometric) (ab117129) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

It can be paired to use withHydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131), which specifically quantifies 5-hmC, for simultaneously quantification of both methylated and hydroxymethylated DNA.

style

left-column

製品内容

{ "values": { "96Test": { "sellingSize": "96 Test", "publicAssetCode":"ab117129-96Test", "assetComponentDetails": [ { "size":"1 x 16 µL", "name":"Fluoro Developer", "number":"AB117129-CMP07" }, { "size":"1 x 20 µL", "name":"Negative Control, 20 µg/mL", "number":"AB117129-CMP10" }, { "size":"1 x 8 mL", "name":"Fluoro Dilutor", "number":"AB117129-CMP08" }, { "size":"12 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB117129-CMP01" }, { "size":"1 x 20 µL", "name":"Positive Control, 20 µg/mL", "number":"AB117129-CMP11" }, { "size":"1 x 16 µL", "name":"Fluoro Enhancer", "number":"AB117129-CMP09" }, { "size":"1 x 16 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB117129-CMP05" }, { "size":"1 x 28 mL", "name":"10X Wash Buffer", "number":"AB117129-CMP02" }, { "size":"1 x 16 µL", "name":"Enhancer Solution", "number":"AB117129-CMP06" }, { "size":"1 x 10 mL", "name":"Binding Solution", "number":"AB117129-CMP03" }, { "size":"1 x 8 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB117129-CMP04" } ] }, "48Test": { "sellingSize": "48 Test", "publicAssetCode":"ab117129-48Test", "assetComponentDetails": [ { "size":"1 x 8 µL", "name":"Detection Antibody, 400 µg/mL", "number":"AB117129-CMP05", "productcode":"" }, { "size":"1 x 8 µL", "name":"Fluoro Developer", "number":"AB117129-CMP07", "productcode":"" }, { "size":"1 x 4 µL", "name":"Capture Antibody, 1000 µg/mL", "number":"AB117129-CMP04", "productcode":"" }, { "size":"1 x 14 mL", "name":"10X Wash Buffer", "number":"AB117129-CMP02", "productcode":"" }, { "size":"1 x 10 µL", "name":"Positive Control, 20 µg/mL", "number":"AB117129-CMP11", "productcode":"" }, { "size":"1 x 10 µL", "name":"Negative Control, 20 µg/mL", "number":"AB117129-CMP10", "productcode":"" }, { "size":"1 x 5 mL", "name":"Binding Solution", "number":"AB117129-CMP03", "productcode":"" }, { "size":"1 x 8 µL", "name":"Enhancer Solution", "number":"AB117129-CMP06", "productcode":"" }, { "size":"6 x 1 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB117129-CMP01", "productcode":"" }, { "size":"1 x 4 mL", "name":"Fluoro Dilutor", "number":"AB117129-CMP08", "productcode":"" }, { "size":"1 x 8 µL", "name":"Fluoro Enhancer", "number":"AB117129-CMP09", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

Multi

長期保存温度

Multi

保管に関する情報

Please refer to protocols

style

left-column

style

left-column

製品プロトコール

style

left-column

ターゲットの情報

See full target information 5-Methylcytosine

style

left-column

文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular bioengineering 18:29-38 PubMed39949487

2025

Matrix Stiffness-Mediated DNA Methylation in Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Paul V Taufalele,Hannah K Kirkham,Cynthia A Reinhart-King

Endocrine connections 6:708-725 PubMed28993426

2017

Effect of diabetes status and hyperglycemia on global DNA methylation and hydroxymethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jairo Arturo Pinzón-Cortés,Angelina Perna-Chaux,Nicolás Steven Rojas-Villamizar,Angélica Díaz-Basabe,Diana Carolina Polanía-Villanueva,María Fernanda Jácome,Carlos Olimpo Mendivil,Helena Groot,Valeriano López-Segura

Toxicology 390:63-73 PubMed28865727

2017

Altered mitochondrial epigenetics associated with subchronic doxorubicin cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

André Ferreira,Teresa Cunha-Oliveira,Rui F Simões,Filipa S Carvalho,Ana Burgeiro,Kendra Nordgren,Kendall B Wallace,Paulo J Oliveira

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Methylated DNA Quantification Kit (Fluorometric)