Methylated DNA Quantification Kit (Colorimetric) (ab117128)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 4 hr
- Sample type: Adherent cells, Plasma, Saliva, Serum, Suspension cells, Tissue, Urine
- Sensitivity: 0.2 ng/well
製品の概要
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製品名
Methylated DNA Quantification Kit (Colorimetric)
5-methylcytosine キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Saliva, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
検出感度
>= 0.2 ng/well -
全工程の試験時間
4h 00m -
種交差性
交差種: Species independent -
製品の概要
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.
Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.
It can be paired to use with Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130), which specifically quantifies 5-hmC, for simultaneous quantification of both methylated and hydroxymethylated DNA.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 48 tests 96 tests 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Binding Solution 1 x 5ml 1 x 10ml Capture Antibody, 1000 µg/ml 1 x 4µl 1 x 8µl Detection Antibody, 400 µg/ml 1 x 8µl 1 x 16µl Developer Solution 1 x 5ml 1 x 10ml Enhancer Solution 1 x 8µl 1 x 16µl Negative Control, 20 µg/ml 1 x 10µl 1 x 20µl Positive Control, 20 µg/ml 1 x 10µl 1 x 20µl Stop Solution 1 x 5ml 1 x 10ml -
研究分野
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別名
- 5 mC
- 5 MethylCytosine
- 5mC
関連製品
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Related Products
画像
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Functional Studies - Methylated DNA Quantification Kit (Colorimetric) (ab117128)Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117128. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117128.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (33)
ab117128 は 33 報の論文で使用されています。
- Meneses-San Juan D et al. Repetitive Transcranial Magnetic Stimulation Reduces Depressive-like Behaviors, Modifies Dendritic Plasticity, and Generates Global Epigenetic Changes in the Frontal Cortex and Hippocampus in a Rodent Model of Chronic Stress. Cells 12:N/A (2023). PubMed: 37626872
- Qaria MA et al. Ectoine Globally Hypomethylates DNA in Skin Cells and Suppresses Cancer Proliferation. Mar Drugs 21:N/A (2023). PubMed: 38132942
- Nur SM et al. Untargeted Metabolomics Showed Accumulation of One-Carbon Metabolites to Facilitate DNA Methylation during Extracellular Matrix Detachment of Cancer Cells. Metabolites 12:N/A (2022). PubMed: 35323710
- Athanasopoulou S et al. Antioxidant and Antiaging Properties of a Novel Synergistic Nutraceutical Complex: Readouts from an In Cellulo Study and an In Vivo Prospective, Randomized Trial. Antioxidants (Basel) 11:N/A (2022). PubMed: 35326118
- Schmidt S et al. Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease. Nat Commun 13:4819 (2022). PubMed: 35974013