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AB117128

Methylated DNA Quantification Kit (Colorimetric)

Methylated DNA Quantification Kit (Colorimetric)

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(38 Publications)

Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

別名を表示する

5 Methycytosine, 5-Me Cytidine, 5-Me Cytosine, 5-Methyl-Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-MethylCytosine, 5-mC, 5-meC, methyl CpG

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Functional Studies - Methylated DNA Quantification Kit (Colorimetric) (AB117128)
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Supplier Data

Functional Studies - Methylated DNA Quantification Kit (Colorimetric) (AB117128)

Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117128. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117128.

Key facts

検出方法

Colorimetric

サンプルタイプ

Saliva, Urine, Plasma, Tissue, Suspension cells, Serum, Adherent cells

検出感度

>= 0.2 ng/well

アッセイ時間

4h

アッセイプラットフォーム

Microplate reader

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製品の詳細

Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

It can be paired to use Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130), which specifically quantifies 5-hmC, for simultaneous quantification of both methylated and hydroxymethylated DNA.

DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.

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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
Multi
長期保存温度
Multi
保管に関する情報
Please refer to protocols
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製品プロトコール

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ターゲットの情報

See full target information 5-Methylcytosine
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文献 (38)

Recent publications for all applications. Explore the full list and refine your search

Biologics : targets & therapy 19:251-264 PubMed40321666

2025

Epigenetic Regulation of Sorbitol Dehydrogenase in Diabetic Retinopathy Patients: DNA Methylation, Histone Acetylation and microRNA-320.

Applications

Unspecified application

Species

Unspecified reactive species

Ramzi Amin,Hikmat Permana,Arief Sjamsulaksan Kartasasmita,Dany Hilmanto,Rachmat Hidayat

Pharmaceutics 16: PubMed39339187

2024

Herbal Formula Extract Ameliorates Anxiety and Cognitive Impairment via Regulation of the Reelin/Dab-1 Pathway in a Murine Model of Post-Traumatic Stress Disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Hee Ra Park,Mudan Cai,Eun Jin Yang

Archives of toxicology 98:2143-2152 PubMed38806716

2024

Patulin alters alpha-adrenergic receptor signalling and induces epigenetic modifications in the kidneys of C57BL/6 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Makabongwe Mazibuko,Terisha Ghazi,Anil Chuturgoon

Frontiers in neuroscience 18:1362497 PubMed38694899

2024

Epigenetic alterations in creatine transporter deficiency: a new marker for dodecyl creatine ester therapeutic efficacy monitoring.

Applications

Unspecified application

Species

Unspecified reactive species

Léa Broca-Brisson,Clémence Disdier,Rania Harati,Rifat Hamoudi,Aloïse Mabondzo

Developmental cell 59:187-198.e7 PubMed38198888

2024

Disruption of H3K36 methylation provokes cellular plasticity to drive aberrant glandular formation and squamous carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Kyung Ko,Amy Anderson,Carina D'souza,Jonathan Zou,Sijia Huang,Sohyun Cho,Faizan Alawi,Stephen Prouty,Vivian Lee,Sora Yoon,Keegan Krick,Yoko Horiuchi,Kai Ge,John T Seykora,Brian C Capell

Marine drugs 21: PubMed38132942

2023

Ectoine Globally Hypomethylates DNA in Skin Cells and Suppresses Cancer Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Majjid A Qaria,Chunyan Xu,Ran Hu,Roua A Alsubki,Mohamed Yassin Ali,Sethupathy Sivasamy,Kotb A Attia,Daochen Zhu

Cells 12: PubMed37626872

2023

Repetitive Transcranial Magnetic Stimulation Reduces Depressive-like Behaviors, Modifies Dendritic Plasticity, and Generates Global Epigenetic Changes in the Frontal Cortex and Hippocampus in a Rodent Model of Chronic Stress.

Applications

Unspecified application

Species

Unspecified reactive species

David Meneses-San Juan,Mónica Lamas,Gerardo Bernabé Ramírez-Rodríguez

Oxidative medicine and cellular longevity 2022:8770136 PubMed36193084

2022

Early Development of Cardiac Fibrosis in Young Old-Father Offspring.

Applications

Unspecified application

Species

Unspecified reactive species

A Ismail,Y Saliba,N Fares

Journal of translational medicine 20:383 PubMed36038893

2022

Low mitochondrial DNA copy number induces chemotherapy resistance via epithelial-mesenchymal transition by DNA methylation in esophageal squamous cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuto Kubo,Koji Tanaka,Yasunori Masuike,Tsuyoshi Takahashi,Kotaro Yamashita,Tomoki Makino,Takuro Saito,Kazuyoshi Yamamoto,Tomoyuki Tsujimoto,Takashi Harino,Yukinori Kurokawa,Makoto Yamasaki,Kiyokazu Nakajima,Hidetoshi Eguchi,Yuichiro Doki

Nature communications 13:4819 PubMed35974013

2022

Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Schmidt,Malte D Luecken,Dietrich Trümbach,Sina Hembach,Kristina M Niedermeier,Nicole Wenck,Klaus Pflügler,Constantin Stautner,Anika Böttcher,Heiko Lickert,Ciro Ramirez-Suastegui,Ruhel Ahmad,Michael J Ziller,Julia C Fitzgerald,Viktoria Ruf,Wilma D J van de Berg,Allert J Jonker,Thomas Gasser,Beate Winner,Jürgen Winkler,Daniela M Vogt Weisenhorn,Florian Giesert,Fabian J Theis,Wolfgang Wurst
View all publications
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組合せ製品

Assay Kits

AB117130

Hydroxymethylated DNA Quantification Kit (Colorimetric)

Functional Studies - Hydroxymethylated DNA Quantification Kit (Colorimetric) (AB117130)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB185912

m6A RNA Methylation Assay Kit (Colorimetric)

ELISA - m6A RNA Methylation Assay Kit (Colorimetric) (AB185912)

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
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