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Maltose Assay Kit (ab83388)

Submit a review Q&A (2)References (1)
Example of Fluorometric Standard Curve
  • Functional Studies - Maltose Assay Kit (ab83388)

Key features and details

  • Assay type: Quantitative
  • Detection method: Colorimetric/Fluorometric
  • Platform: Microplate reader
  • Assay time: 1 hr
  • Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine

製品の概要

  • 製品名

    Maltose Assay Kit

  • 検出方法

    Colorimetric/Fluorometric

  • サンプルの種類

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts

  • アッセイタイプ

    Quantitative

  • 全工程の試験時間

    1h 00m

  • 製品の概要

    Abcam's Maltose Assay Kit provides a fast, easy and sensitive method for quantifying maltose in various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.).


    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    This product is manufactured by BioVision, an Abcam company and was previously called K628 Maltose Colorimetric/Fluorometric Assay Kit. K628-100 is the same size as the 100 test size of ab83388.

    Maltose (C12H22O11; FW: 342.3), one of the main fuel sources to generate the universal energy molecule ATP, is the major disaccharide that is generated from hydrolysis of starch in food. Maltose contains two glucose units joined by a α-1,4-glycosidic linkage, which can be easily converted to two glucoses by α-D-glucosidase. The generated glucose can be specifically oxidized to produce a product that interacts with the probe to generate color and fluorescence. Thus, maltose can be determined by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (Ex/Em = 535/587 nm) methods.

  • 試験プラットフォーム

    Microplate reader

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 100 tests
    a-D-Glucosidase (Lyophilized) 1 vial
    Enzyme Mix (Lyophilized) 1 vial
    Maltose Assay Buffer 1 x 25ml
    Maltose Probe in DMSO 1 x 200µl
    Maltose Standard (100 nmol/µl) 1 x 100µl

  • 研究分野

  • 関連性

    Maltose (C12H22O11; FW: 342.3), one of the main fuel sources to generate the universal energy molecule ATP, is the major disaccharide that is generated from hydrolysis of starch in food.

  • 別名

    • 4 O alpha D Glucopyranosyl D glucose
    • Malt sugar

画像

  • Example of Fluorometric Standard Curve
    Example of Fluorometric Standard Curve

    Maltose with α-D-Glucosidase Standard Curve. Assays were performed following kit instructions

  • Functional Studies - Maltose Assay Kit (ab83388)
    Functional Studies - Maltose Assay Kit (ab83388)
    Maltose Standard Curve. Assays were performed following kit instructions. Open circle is maltose with alpha-D-Glucosidase. Solid square is maltose without alpha-D-Glucosidase. Triangle is free glucose (divided by 2).

参考文献 (1)

ab83388 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab83388 は 1 報の論文で使用されています。

  • Axelsson E  et al. The genomic signature of dog domestication reveals adaptation to a starch-rich diet. Nature N/A:N/A (2013). Functional Studies . PubMed: 23354050

レビューと Q&A

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1-2 of 2 Abreviews or Q&A

Question

Is ethanol extraction of protein samples compatible with use of kit ab83388?

What procedure can be used to extract maltose from tissue samples?

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Answer

Extraction of proteins is not necessary, as kit ab83388 measures maltose. Samples in ethanol are not compatible as the enzymes in the kit work in aqueous solution.

Tissue and cell samples can be homogenized in 2-3 volumes of the Maltose Assay Buffer, and then centrifuged for 5-10 minutes to remove debris; the supernatant is the lysate. Several volumes of this lysate can then be tested to choose a volume or dilution that yields results within the linear range of the standard curve.

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Question

I need to know whether kit signal/color change is based on product formed or product depleted?

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Answer

1) Malate Assay Kit (ab83391): detects product formed.
Step 1: Malate in sample + oxidation = product formed.
Step 2: Product formed + probe = color generation.

2) Maltose Assay Kit (ab83388): detects product formed.
Step 1: Maltose in sample + alpha D glucosidase = two glucose units.
Step 2: Two glucose units + oxidation = product formed. Step 3: Product formed + probe = color generation.

3) Amylase Assay Kit (ab102523): direct detection/amount in sample.
Step 1: Amylase in sample + substrate = fluorescence.

4) Pyruvate Assay Kit (ab65342): detects product formed.
Step 1: Pyruvatein sample + oxidation = product formed.
Step 2: Product formed + probe = color generation.

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