Lysosomal Staining Reagent - Blue - Cytopainter (ab176825)
製品の概要
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製品名
Lysosomal Staining Reagent - Blue - Cytopainter
Lysosomes キット 製品一覧 -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Cell-based (qualitative) -
種交差性
交差種: Mammals, Other species -
製品の概要
Lysosomal Staining Reagent - Blue | Cytopainter (ab176825) is part of a series of new fluorogenic probes to label lysosomes of live cells. The proprietary lysotropic dye selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and becomes trapped in lysosomes after it gets into the cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Review other dyes and kits for lysosomal staining, or the live cell staining fluorescent dyes guide
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特記事項
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5 – 4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains the pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels.
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試験プラットフォーム
Fluorescence microscope
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 500 tests CytoPainter LysoBlue Indicator Reagent 1 x 500 tests -
研究分野
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別名
- lysosome marker
- lysotracker
関連製品
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Corresponding kit
画像
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Image of U2OS cells stained with CytoPainter LysoBlue Indicator Reagent (ab176825)
Image of U2OS cells stained with Abcam's CytoPainter LysoBlue Indicator Reagent (ab176825) in a Costar black wall/clear bottom 96-well plate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab176825 は論文での使用が確認できていません。