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Lipolysis Assay Kit (Fluorometric) (ab185434)

Submit a review Submit a question References (1)
Glycerol Standard Curve.
  • Measurement of glycerol levels in media of 3T3-L1 cells.
  • Inhibition of Isoproterenol stimulated lipolysis.

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Cell culture supernatant
  • Sensitivity: 0.02 nmol/well

製品の概要

  • 製品名

    Lipolysis Assay Kit (Fluorometric)
    Lipolysis キット 製品一覧

  • 検出方法

    Fluorescent

  • サンプルの種類

    Cell culture supernatant

  • アッセイタイプ

    Quantitative

  • 検出感度

    < 0.02 nmol/well

  • 種交差性

    交差種: Mammals, Other species

  • 製品の概要

    Lipolysis Assay Kit (Fluorometric) ab185434 is simple, easy-to-use and highly sensitive.


    This lipolysis assay measures glycerol released from 3T3-L1 cells as early as 1 hour after induction of lipolysis. It is suitable for measuring trace amounts of glycerol from samples. In the assay, glycerol reacts with Glycerol Enzyme Mix to form an intermediate that in turn reacts with Glycerol Developer and PicoProbe™ to generate the fluorescence product. The fluorescence intensity is directly proportional to the amount of glycerol.


    The lipolysis assay kit can detect less than 20 pmol of glycerol.

  • 特記事項

    This product is manufactured by BioVision, an Abcam company and was previously called K578 PicoProbe™ Lipolysis (3T3-L1) Fluorometric Assay Kit. K578-100 is the same size as the 100 test size of ab185434.

    Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue.

  • 試験プラットフォーム

    Microplate reader

製品の特性

画像

  • Glycerol Standard Curve.
    Glycerol Standard Curve.
  • Measurement of glycerol levels in media of 3T3-L1 cells.
    Measurement of glycerol levels in media of 3T3-L1 cells.

    5 μl of 3T3-L1 cells treated with vehicle control (H2O) or 100 nM Isoproterenol for 0-3 hours. Measurements for 0 hours were undetectable.

  • Inhibition of Isoproterenol stimulated lipolysis.
    Inhibition of Isoproterenol stimulated lipolysis.

    Inhibition of Isoproterenol (100 nM) stimulated lipolysis by treatment with 100 nM insulin (measured using 5 μl of media of 3T3-L1 cells). Control was vehicle (H2O) treated cells.

参考文献 (1)

ab185434 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185434 は 1 報の論文で使用されています。

  • Yue F  et al. Lipid droplet dynamics regulate adult muscle stem cell fate. Cell Rep 38:110267 (2022). PubMed: 35045287

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