L-Lactate Assay Kit (Colorimetric) (ab65331)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture supernatant, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 0.02 mM
製品の概要
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製品名
L-Lactate Assay Kit (Colorimetric)
L-Lactate キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate -
アッセイタイプ
Quantitative -
検出感度
0.02 mM -
検出範囲
0.02 mM - 10 mM -
全工程の試験時間
0h 30m -
製品の概要
L-Lactate Assay Kit (Colorimetric) (ab65331) uses an assay protocol where lactate is oxidized by lactate dehydrogenase to generate a product which interacts with a probe to produce a color (λmax = 450 nm).
The kit detects L(+)-Lactate in biological samples such as serum or plasma, cells, tissues, cell culture and fermentation media.
Lactate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at room temp
- analyze with microplate reader -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K627 Lactate Colorimetric Assay Kit II. K627-100 is the same size as the 100 test size of ab65331.
L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present (see D-Lactate assay kits) but only at about 1-5% of the concentration of L(+)-Lactate.
L-Lactate assay kit ab65331 is our most popular L-Lactate assay kit (colorimetric 450nm, range 0.02 mM - 10 mM). Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range:
- L-Lactate assay ab65330: colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM
- L-Lactate assay ab169557: fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.How other researchers have used L-Lactate Assay Kit ab65331
This Lactate assay kit has been used in publications in a variety of sample types, including:
- Human: THP-1 cell lysates1, MDA-MB-231 and HepG2 cell culture lysates2, cell culture supernatant (HepG2, A549, Huh7, PC3, LN229, HeLa)3, brain tissue4
- Mouse: brown adipose tissue lysate5, thymic lymphoma tissue6, cell culture supernatant7, T cell primary cell culture supernatants8, serum9, serum and muscle10
- Bovine: cumulus cell culture supernatant11References: 1 - Tran UT and Kitami T 2019; 2 - Cui J et al 2019; 3 - Rodriguez ML et al 2018, Chen Y et al 2018, Zhang D et al 2018, Caino MC et al 2017, Birkenmeier K et al 2015; 4 - Sullivan RC et al 2019; 5 - Jeong JH et al 2018; 6 - Vara-Ciruelos D et al 2019; 7 - Fiorenzano et al 2016; 8 - Menk AV et al 2018; 9 - Deng W et al 2019, Guglielmetti C et al 2017, Kang R et al 2016; 10 - Kim HY et al 2016; 11 - Sinha et al 2017
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests L(+)-Lactate Standard 1 x 100µl Assay Buffer XII 1 x 25ml Enzyme Mix XV 1 vial Developer Solution III 1 vial -
研究分野
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関連性
Lactate (CH3CH(OH)COO-) plays important roles in many biological processes. Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereoisomer of lactate formed in human intermediary metabolism and is present in blood. The lactate to pyruvate ratio reflects the redox state of the cell and describes the balance beween NAD+ and NADH, which is dependent on the interconversion of lactate and pyruvate via lactate dehydrogenase (LDH).
関連製品
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Related Products
画像
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L-Lactate Assay Kit (Colorimetric) ab65331Image from Kelly CE et al., PLoS Biol 11(4), fig2d. oi: 10.1371/journal.pbio.1001538. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Plasma lactate concentrations were determined using L-Lactate assay kit (ab65331) in Ark2C+/+ and Ark2C−/− (Arkadia-like gene) mice.
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ab65331 L-Lactate Assay Kit (Colorimetric)Linearity of dilution: concentration of L-Lactate in differently diluted (X-axis) biological samples, demonstrating a linearity of 89%-111% (concentrations corrected for by factor of dilution; duplicates; +/- SD).
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L-Lactate Assay Kit (Colorimetric) (ab65331)Relative signal (RFU) in unfiltered human plasma (dilution 1:8), comparing L-lactate signals with background reading (no enzyme) after 10 minutes of incubation (duplicates +/- SD).
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ab65331 L-Lactate Assay Kit (Colorimetric)Standard curve with background signal subtracted (duplicates; +/- SD).
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L-Lactate Assay Kit (Colorimetric) (ab65331)Lactate Standard Curve. The assay is performed following the kit (ab65331) protocol.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (272)
ab65331 は 272 報の論文で使用されています。
- Guo C et al. HIF-1α accumulation in response to transient hypoglycemia may worsen diabetic eye disease. Cell Rep 42:111976 (2023). PubMed: 36640318
- Li Z et al. IRF7 inhibits the Warburg effect via transcriptional suppression of PKM2 in osteosarcoma. Int J Biol Sci 18:30-42 (2022). PubMed: 34975316
- Wang J et al. DNAJC12 activated by HNF1A enhances aerobic glycolysis and drug resistance in non-small cell lung cancer. Ann Transl Med 10:492 (2022). PubMed: 35571408
- Wang W & Wang B KDM3A-mediated SP1 activates PFKFB4 transcription to promote aerobic glycolysis in osteosarcoma and augment tumor development. BMC Cancer 22:562 (2022). PubMed: 35590288
- Russell S et al. Proton export upregulates aerobic glycolysis. BMC Biol 20:163 (2022). PubMed: 35840963