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AB241028

L-Arginine Assay Kit

L-Arginine Assay Kit

  • JP Deleterious:医薬用外劇物

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(5 Publications)

L-Arginine Assay Kit (ab241028) provides a quick, specific, and easy method for the measurement of total L-arginine concentrations in a wide variety of samples.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
3 Images
Enzyme activity assay - L-Arginine Assay Kit (AB241028)
  • Enzyme activity assay

Unknown

Enzyme activity assay - L-Arginine Assay Kit (AB241028)

Estimations of L-arginine in two human serum sample (30 µL) and orange juice (1.6 µL).

Estimations of L-arginine in two human serum sample (30 μL) and orange juice (1.6 μL). L-arginine concentrations were 0.278 mM and 0.069 mM in the two human serum samples, and 5.113 mM in orange juice. Assays were performed following the kit protocol.

Enzyme activity assay - L-Arginine Assay Kit (AB241028)
  • Enzyme activity assay

Unknown

Enzyme activity assay - L-Arginine Assay Kit (AB241028)

Specificity of the detection of L-arginine over other amino acids : D-arginine and other L-amino acids were tested at a 2-fold molar excess (each AA : 40 nmol) vs L-arginine (20 nmol).

Enzyme activity assay - L-Arginine Assay Kit (AB241028)
  • Enzyme activity assay

Unknown

Enzyme activity assay - L-Arginine Assay Kit (AB241028)

L-arginine standard curve.

Key facts

検出方法

Colorimetric

サンプルタイプ

Other biological fluids, Drinks

製品の詳細

L-Arginine Assay Kit (ab241028) provides a quick, specific, and easy method for the measurement of total L-arginine concentrations in a wide variety of samples. In this enzyme-based assay, L-arginine is converted into a series of intermediates, which will further react with a probe producing a stable colorimetric signal (OD: 450 nm).

The kit is simple to use, sensitive and high-throughput adaptable and can detect as low as 1 nmol/well of L-arginine in biological samples.

Arginine assay protocol summary
- add samples and standards to wells- add enzyme mix and background mix, and incubate for 30-min at 37°C- add reaction mix, and incubate for 60-min at 37°C- analyse with plate reader

Other notes
This product was previously called K749 Biovision L-Arginine Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

製品内容

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab241028-100Test", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Probe V", "number":"AB241028-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Arginine Probe Mix B", "number":"AB241028-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Converter Mix D", "number":"AB241028-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 5", "number":"AB241028-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Arginine Enzyme Mix", "number":"AB241028-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Arginine", "number":"AB241028-CMP05", "productcode":"" } ] } } }

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
保管に関する情報
Please refer to protocols

製品プロトコール

ターゲットの情報

See full target information Arginine

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 40:1-18 PubMed38873262

2024

Mitochondria-engine with self-regulation to restore degenerated intervertebral disc cells via bioenergetic robust hydrogel design.

Applications

Unspecified application

Species

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Juehan Wang,Yulin Jiang,Ce Zhu,Zheng Liu,Lin Qi,Hong Ding,Jing Wang,Yong Huang,Yubao Li,Yueming Song,Ganjun Feng,Li Zhang,Limin Liu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2307256 PubMed38233193

2024

Role of Argininosuccinate Synthase 1 -Dependent L-Arginine Biosynthesis in the Protective Effect of Endothelial Sirtuin 3 Against Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Cao,Vivian Wei Yan Wu,Yumeng Han,Huiling Hong,Yalan Wu,Alice Pik Shan Kong,Kathy O Lui,Xiao Yu Tian

Frontiers in endocrinology 14:1252727 PubMed37810891

2023

Potential involvement of the bone marrow in experimental Graves' disease and thyroid eye disease.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Gulbins,Mareike Horstmann,Simone Keitsch,Matthias Soddemann,Barbara Wilker,Gregory C Wilson,Ryan Zeidan,Gary D Hammer,Anke Daser,Nikolaos E Bechrakis,Gina-Eva Görtz,Anja Eckstein

Oxidative medicine and cellular longevity 2021:5510663 PubMed33791071

2021

Pegylated Recombinant Human Arginase 1 Induces Autophagy and Apoptosis via the ROS-Activated AKT/mTOR Pathway in Bladder Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuyun Zhao,Peng Zhang,Wei Li,Dengchuan Wang,Changneng Ke,Yueming Liu,James Chung-Man Ho,Paul Ning-Man Cheng,Shi Xu

Communications biology 3:611 PubMed33097830

2020

Exogenous L-arginine increases intestinal stem cell function through CD90+ stromal cells producing mTORC1-induced Wnt2b.

Applications

Unspecified application

Species

Unspecified reactive species

Qihang Hou,Yuanyang Dong,Jingxi Huang,Chaoyong Liao,Jiaqi Lei,Youli Wang,Yujiao Lai,Yifei Bian,Yang He,Jingjing Sun,Meng Sun,Qiuyu Jiang,Bo Wang,Zhengquan Yu,Yuming Guo,Bingkun Zhang
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