AB102528

Isocitrate Dehydrogenase Assay Kit (Colorimetric)

Name: Isocitrate Dehydrogenase Assay Kit (Colorimetric) (ab102528) | Abcam
Brand: Abcam Limited
SKU: AB102528
Rating: 4 (1 reviews)

(1 Review)

(25 Publications)

Isocitrate Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of NAD(+) / NADP(+)-dependent or both IDHs in a variety of samples.

別名を表示する

PICD, IDH1, Isocitrate dehydrogenase [NADP] cytoplasmic, IDH, Cytosolic NADP-isocitrate dehydrogenase, IDPc, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase

2 Images

Functional Studies - Isocitrate Dehydrogenase Assay Kit (Colorimetric) (AB102528)

FuncS

Supplier Data

Functional Studies - Isocitrate Dehydrogenase Assay Kit (Colorimetric) (AB102528)

Example of NADH and NADPH Standard Curve obtained using ab102528

FuncS

Supplier Data

Functional Studies - Isocitrate Dehydrogenase Assay Kit (Colorimetric) (AB102528)

Isocitrate Dehydrogenase detection in Bovine Liver Extraction Sample using ab102528

Key facts

検出方法

Colorimetric

サンプルタイプ

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

アッセイタイプ

Enzyme activity

検出感度

> 0.01 mU/well

アッセイ時間

45m

アッセイプラットフォーム

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's Isocitrate Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of NAD(+) / NADP(+)-dependent or both IDHs in a variety of samples. The IDHs utilize isocitrate as a specific substrate leading to a proportional color development and can be easily quantified colorimetrically (λ = 450 nm) with detection sensitivity as low as 0.01 mU.

Visit our FAQs page for tips and troubleshooting.

Isocitrate dehydrogenase assay protocol summary:
- add samples and standards to wells
- incubate for 3 min and analyze with microplate reader
- incubate for 30 min - 2 hr and analyze again

This product is manufactured by BioVision, an Abcam company and was previously called K756 Isocitrate Dehydrogenase Activity Colorimetric Assay Kit. K756-100 is the same size as the 100 test size of ab102528.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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製品内容

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab102528-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"NAD+", "number":"AB102528-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"Isocitrate Assay Buffer", "number":"AB102528-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"IDH Substrate Mix", "number":"AB102528-CMP04", "productcode":"" }, { "size":"1 x 20 µL", "name":"IDH Positive Control", "number":"AB102528-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Beta-NADP Stock", "number":"AB102528-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution II", "number":"AB102528-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"NADH Standard I", "number":"AB102528-CMP07", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Isocitrate Dehydrogenase often referred to as IDH includes multiple isoforms such as IDH1 and IDH2. These enzymes catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (α-KG) producing NADPH from NADP+ in the process. IDH1 weighs approximately 46 kDa and is located in the cytoplasm and peroxisomes while IDH2 operates in the mitochondria. Both isoforms of IDH are essential in cellular metabolism contributing to the citric acid cycle.

Biological function summary

Isocitrate conversion catalyzed by IDH maintains the balance of cellular NADP+/NADPH which is important for protecting cells from oxidative stress. NADPH acts as a reducing agent in several anabolic reactions such as fatty acid and nucleotide synthesis. IDH does not function as part of a large complex but its action significantly impacts redox homeostasis within cells.

Pathways

IDH plays a critical role in the citric acid cycle converting isocitrate to α-KG an important intermediate. This reaction connects to multiple metabolic pathways that influence energy production. Furthermore the production of α-KG links IDH activity with related proteins like α-Ketoglutarate dehydrogenase and glutamate dehydrogenase which further process metabolic intermediates in the cycle.

Mutations in the IDH1 and IDH2 genes link to various cancers such as glioma and acute myeloid leukemia (AML). These mutations often cause a neomorphic enzyme activity producing 2-hydroxyglutarate instead of α-KG which disrupts cellular metabolism and contributes to oncogenesis. The abnormal 2-hydroxyglutarate production interrelates with proteins involved in epigenetic regulation affecting DNA and histone methylation states which are frequently altered in cancerous cells.

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製品プロトコール

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ターゲットの情報

Catalyzes the NADP(+)-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase (PubMed : 10521434, PubMed : 19935646). Plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways (PubMed : 10521434). May act as a corneal epithelial crystallin and may be involved in maintaining corneal epithelial transparency (By similarity).

See full target information IDH1

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文献 (25)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:111833 PubMed39949963

2025

Adenylate cyclase 10 promotes brown adipose tissue thermogenesis.

Applications

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Species

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Anupam Das,Christine Mund,Eman Hagag,Ruben Garcia-Martin,Eleftheria Karadima,Anke Witt,Mirko Peitzsch,Andreas Deussen,Triantafyllos Chavakis,Thomas Noll,Vasileia Ismini Alexaki

Nature communications 15:9945 PubMed39550366

2024

NOTCH1 mitochondria localization during heart development promotes mitochondrial metabolism and the endothelial-to-mesenchymal transition in mice.

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Unspecified application

Species

Unspecified reactive species

Jie Wang,Rui Zhao,Sha Xu,Xiang-Yu Zhou,Ke Cai,Yu-Ling Chen,Ze-Yu Zhou,Xin Sun,Yan Shi,Feng Wang,Yong-Hao Gui,Hui Tao,Jian-Yuan Zhao

Frontiers in molecular biosciences 11:1460987 PubMed39297074

2024

Recent progress of methods for cuproptosis detection.

Applications

Unspecified application

Species

Unspecified reactive species

Ligang Zhang,Ruiting Deng,Raoqing Guo,Yawen Jiang,Yichen Guan,Caiyue Chen,Wudi Zhao,Guobin Huang,Lian Liu,Hongli Du,Dongsheng Tang

Cancer discovery 14:1922-1939 PubMed38787341

2024

Targeting Acute Myeloid Leukemia Stem Cells through Perturbation of Mitochondrial Calcium.

Applications

Unspecified application

Species

Unspecified reactive species

Anagha Inguva Sheth,Mark J Althoff,Hunter Tolison,Krysta Engel,Maria L Amaya,Anna E Krug,Tracy N Young,Mohammad Minhajuddin,Shanshan Pei,Sweta B Patel,Amanda Winters,Regan Miller,Ian T Shelton,Jonathan St-Germain,Tianyi Ling,Courtney L Jones,Brian Raught,Austin E Gillen,Monica Ransom,Sarah Staggs,Clayton A Smith,Daniel A Pollyea,Brett M Stevens,Craig T Jordan

Signal transduction and targeted therapy 9:105 PubMed38679634

2024

A positive feedback inhibition of isocitrate dehydrogenase 3β on paired-box gene 6 promotes Alzheimer-like pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wang,Qian Liu,Hai-Tao Yu,Jia-Zhao Xie,Jun-Ning Zhao,Zhi-Ting Fang,Min Qu,Yao Zhang,Ying Yang,Jian-Zhi Wang

Nature communications 14:4360 PubMed37468519

2023

Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanism.

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Unspecified application

Species

Unspecified reactive species

Shubhi Srivastava,Priyanka Gajwani,Jordan Jousma,Hiroe Miyamoto,Youjeong Kwon,Arundhati Jana,Peter T Toth,Gege Yan,Sang-Ging Ong,Jalees Rehman

eLife 12: PubMed37449973

2023

Succinate mediates inflammation-induced adrenocortical dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Ivona Mateska,Anke Witt,Eman Hagag,Anupam Sinha,Canelif Yilmaz,Evangelia Thanou,Na Sun,Ourania Kolliniati,Maria Patschin,Heba Abdelmegeed,Holger Henneicke,Waldemar Kanczkowski,Ben Wielockx,Christos Tsatsanis,Andreas Dahl,Axel Karl Walch,Ka Wan Li,Mirko Peitzsch,Triantafyllos Chavakis,Vasileia Ismini Alexaki

Antioxidants (Basel, Switzerland) 12: PubMed36978833

2023

IDH2 Deficiency Promotes Endothelial Senescence by Eliciting miR-34b/c-Mediated Suppression of Mitophagy and Increased ROS Production.

Applications

Unspecified application

Species

Unspecified reactive species

Ikjun Lee,Shuyu Piao,Seonhee Kim,Harsha Nagar,Su-Jeong Choi,Minsoo Kim,Giang-Huong Vu,Byeong-Hwa Jeon,Cuk-Seong Kim

Nature cancer 3:852-865 PubMed35681100

2022

Limited nutrient availability in the tumor microenvironment renders pancreatic tumors sensitive to allosteric IDH1 inhibitors.

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Unspecified application

Species

Unspecified reactive species

Ali Vaziri-Gohar,Joel Cassel,Farheen S Mohammed,Mehrdad Zarei,Jonathan J Hue,Omid Hajihassani,Hallie J Graor,Yellamelli V V Srikanth,Saadia A Karim,Ata Abbas,Erin Prendergast,Vanessa Chen,Erryk S Katayama,Katerina Dukleska,Imran Khokhar,Anthony Andren,Li Zhang,Chunying Wu,Bernadette Erokwu,Chris A Flask,Mahsa Zarei,Rui Wang,Luke D Rothermel,Andrea M P Romani,Jessica Bowers,Robert Getts,Curtis Tatsuoka,Jennifer P Morton,Ilya Bederman,Henri Brunengraber,Costas A Lyssiotis,Joseph M Salvino,Jonathan R Brody,Jordan M Winter

Communications biology 5:467 PubMed35577894

2022

Mitochondrial TrxR2 regulates metabolism and protects from metabolic disease through enhanced TCA and ETC function.

Applications

Unspecified application

Species

Unspecified reactive species

E Sandra Chocron,Kennedy Mdaki,Nisi Jiang,Jodie Cropper,Andrew M Pickering

View all publications

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Abcam product promise

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Isocitrate Dehydrogenase Assay Kit (Colorimetric)