Iron Assay Kit (Colorimetric) (ab83366)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Hep Plasma, Other biological fluids, Serum, Urine
- Sensitivity: 8 µM
製品の概要
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製品名
Iron Assay Kit (Colorimetric)
Iron キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Urine, Serum, Other biological fluids, Hep Plasma -
アッセイタイプ
Quantitative -
検出感度
> 8 µM -
検出範囲
8 µM - 400 µM -
全工程の試験時間
1h 30m -
製品の概要
Iron Assay Kit ab83366 provides a simple convenient means of measuring ferrous (Fe2+) and/or ferric (Fe3+) iron in biological samples.
How the assay works
This ferrozine assay kit / iron assay kit uses an improved variant of the ferrozine molecule (ferene) which has been shown to give more accurate results for biological iron levels; that are closer to the results seen with mass spectrometry.
In a ferrozine assay, free ferrous iron (Fe2+) reacts with the ferrozine molecule (in this case the ferene molecule) to produce a stable colored complex with absorbance that can be measured with a colorimetric plate reader. The ferene iron probe used in this assay kit has absorbance at 593 nm when complexed with ferrous iron (Fe2+).
In Iron Assay Kit ab83366, in addition to the ferene iron probe and iron standard, an acidic assay buffer, with pH less than 5.5, is provided to release iron from iron carrier proteins. A reducing agent is also provided to convert ferric iron (Fe3+) to form Fe2+.
Ferrous iron (Fe2+) can be measured alone, or by addition of the reducing agent the level of total iron (Fe2+ and Fe3+) can be measured. The level of ferric iron (Fe3+) is calculated by subtracting ferrous iron from total iron.
A specific chelate chemical is included in the buffer to block copper ion (Cu2+) interference.
The kit measures iron in the linear range of 0.4 to 10 nmol or 8 μM to 200 μM.
Iron assay protocol summary
- Add samples and standards to wells
- For Fe2+ assay add assay buffer only, for total iron (Fe2+ and Fe3+) add iron reducer
- Incubate for 30 min at 37°C
- Add Iron Probe and incubate for 60 min at 37°C
- Analyze with microplate reader
Getting the best performance from ab83366
This assay is not suitable for use with samples containing EDTA or citrate.
Related and recommended products
Iron Assay Kit ab83366 is often used in combination with Lipid Peroxidation (MDA) Assay Kit ab118970 and GSH/ GSSG Assay Kit ab239709 in the study of ferroptosis.
To measure total iron binding capacity and iron levels in serum, we recommend Total Iron-Binding Capacity (TIBC) and Serum Iron Assay Kit ab239715.
For iron staining in tissues, we recommend Iron Stain Kit (Prussian Blue Stain) ab150674.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K390 Iron Colorimetric Assay Kit. K390-100 is the same size as the 100 test size of ab83366. -
特記事項
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2000 tests Iron Assay Buffer 1 x 25ml 20 x 25ml Iron Probe 1 x 12ml 20 x 12ml Iron Reducer 1 x 700µl 20 x 700µl Iron Standard I 1 x 100µl 20 x 100µl -
研究分野
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関連性
Iron is essential to nearly all known organisms. It is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Inorganic iron also contributes to redox reactions in the iron-sulfur clusters of many enzymes, such as nitrogenase and hydrogenase. -
別名
- Fe
- Fe++
- Fe+++
画像
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Functional studies - ab83366Leal SM Jr et al., PLoS Pathog 9(7), fig 2c. doi: 10.1371/journal.ppat.1003436. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Leal SM Jr et al examined if iron availability regulates fungal growth in an infection as fungal infection intiates an iron sequestration response. Mice given Fe-dextran (Fe-Dex) and deferoxamine (Defox) shows a higher fungal mass (Fungal dsRed) compared to vehicle treated mice over 48 hours. Iron content was quantified in mouse serum using Iron assay kit (ab83366).
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Functional Studies - Iron Assay Kit (ab83366)Iron measured in human urine showing concentration (micromolar)
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Functional Studies - Iron Assay Kit (ab83366)Assay of soluble free iron from a soil sample (5 μL of 100 μL buffer into which 100 mg of soil had been stirred), 5 μL of FBS and 5 μL of a 100 μM sample of iron standard.
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Functional Studies - Iron Assay Kit (ab83366)Example of iron standard curve using ab83366.
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Functional Studies - Iron Assay Kit (ab83366)Assay of total iron(II + III) in off-the-clot human serum (50 µl of serum was added per well). Data are mean ± SEM from 2 independent replicates, performed in duplicate wells.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (367)
ab83366 は 367 報の論文で使用されています。
- Ma Y et al. Echinacoside ameliorates doxorubicin‑induced cardiac injury by regulating GPX4 inhibition‑induced ferroptosis. Exp Ther Med 27:29 (2024). PubMed: 38125366
- Hu Y et al. Serine and arginine rich splicing factor 1‑regulated microtubule interacting and trafficking domain containing 1 affects colorectal cancer progression and ferroptosis. Exp Ther Med 27:45 (2024). PubMed: 38144919
- Pang P et al. Knockdown of fat mass and obesity alleviates the ferroptosis in Parkinson's disease through m6A-NRF2-dependent manner. Cell Biol Int 48:431-439 (2024). PubMed: 38180302
- Xu X et al. SENP1 inhibits ferroptosis and promotes head and neck squamous cell carcinoma by regulating ACSL4 protein stability via SUMO1. Oncol Rep 51:N/A (2024). PubMed: 38186303
- Wang J et al. POU6F1 promotes ferroptosis by increasing lncRNA-CASC2 transcription to regulate SOCS2/SLC7A11 signaling in gastric cancer. Cell Biol Toxicol 40:3 (2024). PubMed: 38267746