Histone H3 (K27) Methyltransferase Activity Quantification Assay Kit (ab113454)

Thank you for contacting us.

I am sorry but the compositon of the components in our Episeeker Histone H3 (K27) Methytransferase Activity kit and ab113474 Episeker Nuclear Extraction kits are considered proprietary and I am unable to share that information. I am sorry for any inconvenience this may cause.

While I could not provide the information request this time, please do not hesitate to contact us if you need any more advice or information.

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The strip wells are coated with streptavidin but unfortunately they are not available separately.

Thank you for your phone call.

We will continue to guarantee all of our products, even if they were at room temperature for several days due to shipping issues. For this kit in particular, I would expect it to still work normally even though it has been at room temperature for 72 hours. If you do not see a linear standard curve, that would indicate that one or more of the components is not functioning properly and we will be happy to replace the kit for you.


I hope this helps. If you or your student have any further questions, please let me know and I will be happy to help you further.

Thank you for contacting us.

I am sorry to hear the kit is not quite working as expected.

Could you please send me the following information in order for us to evaluate this problem:

1) The data you obtained for the standard curve ( raw data and graph, if possible)
2) The data for your samples
3) Any potential deviations from the protocol that was provided in terms of sample preparation and procedure.
4) What was your lot number and order or PO number?

I look forward to hear back from you and assist you in resolving this case.

I received an update from the developer of the kit shortly after I sent my previous e-mail to you. The information they provided basically confirms what I had suggested in my e-mail, except that sonication will apparently not diminish activity: 1. Yes, these are both typos, they should be lysine 27. We apologize for any inconvenience caused by this. 2. 3 X 10 sec sonication at 4C will not affect enzyme activity. 3. It is about 11000 G (12000 rpm. Rotor radius = 7 cm for bench-top centrifuge such as Eppendorf 5415C). 4. The nuclear extract prepared with the kit is successfully used for WB histone detection. 5. The DTT solution is specifically formulated to be stored at 4C. It is stable at 4C for at least 6 months. Appearance of a very little precipitates is normal. It may be seen when storage temperature is <4C. Please contact me if you still have question or concerns.

Thank you for your inquiry. Compared to similar kits that use conventional nuclear extraction methods,  the buffer included in our nuclear extraction kit (ab 113474) contains much lower amounts of salts (20% of conventional kits) and no SDS, which would allow the enzyme activity to be better kept in the nuclear extracts.   I hope this information helps. Please contact me with any other questions.