GST Activity Assay Kit (Fluorometric) (ab65325)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Other biological fluids, Plasma, Purified protein, Saliva, Serum, Tissue Lysate, Urine
- Sensitivity: 0.5 mU/well
製品の概要
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製品名
GST Activity Assay Kit (Fluorometric)
GST キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Saliva, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Purified protein, Tissue Lysate -
アッセイタイプ
Enzyme activity -
検出感度
< 0.5 mU/well -
全工程の試験時間
2h 00m -
製品の概要
Abcam's GST Activity Assay Kit (Fluorometric) provides a simple, fluorescence-based in vitro assay for detecting the GST activity using fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces in blue (Ex/Em = 380/461 nm) after reaction with glutathione catalysed by GST. The change in fluorescence overtime allows for an easy measurement of the sample GST level.
The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein.
Visit our FAQs page for tips and troubleshooting. -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K260 GST Fluorometric Activity Assay Kit. K260-100 is the same size as the 100 test size of ab65325.
Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests GSH Substrate 2 vials GST Assay Buffer 1 x 25ml GST Enzyme Standard 1 x 10µl MCB Substrate 1 x 200µl -
研究分野
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関連性
Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. -
細胞内局在
Cytoplasmic -
別名
- Glutathione S transferase A1
- Glutathione S transferase A2
- GST class alpha 1
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関連製品
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Related Products
画像
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Functional Studies - GST Activity Assay Kit (Fluorometric) (ab65325)Standard Calibration Curve of GST Measured by Fluorometry. Various amounts of standard GST were incubated with GSH and MCB according to the kit instructions. Fluorescence was measured at ex/em 380/460 nm.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab65325 は 3 報の論文で使用されています。
- Martens GA et al. Elevated antioxidant defence in the brain of deep-diving pinnipeds. Front Physiol 13:1064476 (2022). PubMed: 36589435
- Peng L et al. Inhibition of glutathione metabolism attenuates esophageal cancer progression. Exp Mol Med 49:e318 (2017). PubMed: 28428633
- Plieskatt JL et al. Advances in vaccines against neglected tropical diseases: enhancing physical stability of a recombinant hookworm vaccine through biophysical and formulation studies. Hum Vaccin Immunother 8:765-76 (2012). PubMed: 22495115