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Granzyme B Inhibitor Screening Assay Kit (ab157405)

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Inhibition of Granzyme B Activity

    Key features and details

    • Assay type: Quantitative
    • Detection method: Fluorescent
    • Platform: Microplate reader

    製品の概要

    • 製品名

      Granzyme B Inhibitor Screening Assay Kit
      Granzyme B キット 製品一覧

    • 検出方法

      Fluorescent

    • アッセイタイプ

      Quantitative

    • 製品の概要

      In Abcam's Granzyme B Inhibitor Screening Assay Kit, active human Granzyme B enzyme hydrolyzes the specific substrate to release the quench of fluorescent group, which can be detected fluorometrically at Ex/Em = 380/500 nm. In the presence of potent Granzyme B Inhibitor, the hydrolyzation of substrate will be impeded. The Kit provides a rapid, simple, sensitive and reliable test suitable for high throughput screening of Granzyme B inhibitors or characterize/study Granzyme B inhibitors. Inhibitor control, Ac-IEPD-CHO is included to compare the efficacy of test inhibitors.

    • 特記事項

      This product is manufactured by BioVision, an Abcam company and was previously called K169 Granzyme B Inhibitor Screening Kit (Fluorometric). K169-100 is the same size as the 100 test size of ab157405.

      Granzyme B (GZMB, EC number 3.4.21.79) also known as Granzyme-2 is a serine protease stored in the granules of activated cytotoxic T cells and NK cells. Upon target cell contact, Granzyme B is directionally exocytosed and with the assistance of perforin enters the target cell. With its unique substrate specificity (Granzyme B prefers an aspartic acid residue at the P1 site of its substrates), Granzyme B processes and activates various pro-caspases thereby inducing apoptosis in the target cell.

      The standard is affinity purified.

    • 試験プラットフォーム

      Microplate reader

    製品の特性

    • 保存方法

      Store at -20°C. Please refer to protocols.
    • 内容 100 tests
      Granzyme B Assay Buffer 1 x 25ml
      Granzyme B Enzyme 1 vial
      Granzyme B Substrate 1 x 0.5ml
      Inhibitor Control (Ac-IEPD-CHO) 1 x 20µl

    • 研究分野

    • 機能

      This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

    • 配列類似性

      Belongs to the peptidase S1 family. Granzyme subfamily.
      Contains 1 peptidase S1 domain.

    • 細胞内局在

      Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells.
    • Information by UniProt

    • 別名

      • C11
      • Cathepsin G like 1
      • Cathepsin G-like 1
      • CCPI
      • CGL 1
      • CGL1
      • CSP B
      • CSPB
      • CTLA 1
      • CTLA-1
      • CTLA1
      • CTSGL1
      • Cytotoxic serine protease B
      • Cytotoxic T lymphocyte associated serine esterase 1
      • Cytotoxic T lymphocyte proteinase 2
      • Cytotoxic T-lymphocyte proteinase 2
      • Fragmentin 2
      • Fragmentin-2
      • GRAB_HUMAN
      • Granzyme 2
      • Granzyme B
      • Granzyme B (granzyme 2, cytotoxic T lymphocyte associated serine esterase 1)
      • Granzyme-2
      • GranzymeB
      • GRB
      • Gzmb
      • Hlp
      • Human lymphocyte protein
      • Lymphocyte protease
      • Protease, serine, B
      • SECT
      • T cell serine protease 1 3E
      • T cell serine protease 1-3E
      • T-cell serine protease 1-3E
      see all

    画像

    • Inhibition of Granzyme B Activity
      Inhibition of Granzyme B Activity
      Inhibition of Granzyme B Activity by Granzyme B Inhibitor, Ac-IEPD-CHO. Assay was performed following Kit protocol.

    参考文献 (0)

    ab157405 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab157405 は論文での使用が確認できていません。

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