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Glutathione Reductase (GR) Assay Kit (ab83461)

Submit a review Q&A (3)References (35)
Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Colorimetric
  • Platform: Microplate reader
  • Assay time: 40 min
  • Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
  • Sensitivity: 0.1 mU/ml

製品の概要

  • 製品名

    Glutathione Reductase (GR) Assay Kit

  • 検出方法

    Colorimetric

  • サンプルの種類

    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate

  • アッセイタイプ

    Enzyme activity

  • 検出感度

    > 0.1 mU/ml

  • 検出範囲

    0.1 mU/ml - 40 mU/ml

  • 全工程の試験時間

    0h 40m

  • 製品の概要

    Glutathione Reductase Assay Kit (ab83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.


    Since Glutathione Reductase has significantly higher concentrations in cells (mM range) compared to Thioredoxin Reductase (µM range), we predict that ab83461 will detect mostly GR activity in samples.

  • 特記事項

    This product is manufactured by BioVision, an Abcam company and was previously called K761 Glutathione Reductase Activity Colorimetric Assay Kit. K761-200 is the same size as the 200 test size of ab83461.

    Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.

    Related products

    Review the oxidative stress marker and assay guide to learn about more assays for oxidative stress.

  • 試験プラットフォーム

    Microplate reader

製品の特性

画像

  • Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase (GR) Assay Kit (ab83461)Cao, Zeyu et al., Scientific reports?vol. 7,1 16278., Fig 5, doi:10.1038/s41598-017-16446-7
    ICAC decreased the ROS level induced by EV71 infection via modulating the antioxidant enzymes involved in GSH metabolism. Infected (100 TCID50 EV71) Vero cells were treated with medium or 100 µM ICAC for 12 h. Uninfected cells were used as the control group. The antioxidant enzymes activities of the Vero cells were detected (n = 3). All results were expressed as the means ± SEs. Asterisks indicate that the data significantly differ from the EV71 group at the P < 0.05 level according to one-way analysis of variance.
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)

    Glutathione reductase measured in mouse tissue lysates showing activity (mU) per mg of extracted protein (T1=2 min; T2=30 min).

  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)

    Glutathione reductase measured in cell lysates (mU) per 106 cells (T1=2 min; T2=30 min)

  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)

    Glutathione reductase measured in human plasma and serum (T1=2 min; T2=30 min) showing activity (mU) per ml of tested sample. No activity was detected in saliva or urine.

  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Glutathione Reductase assay time line using ab83461.
  • Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    Functional Studies - Glutathione Reductase Assay Kit (ab83461)
    TNB Standard Curve using ab83461.

参考文献 (35)

ab83461 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab83461 は 35 報の論文で使用されています。

  • Lee J  et al. Quercetin-Induced Glutathione Depletion Sensitizes Colorectal Cancer Cells to Oxaliplatin. Foods 12:N/A (2023). PubMed: 37107528
  • Ureta-Velasco N  et al. Human Milk Composition and Nutritional Status of Omnivore Human Milk Donors Compared with Vegetarian/Vegan Lactating Mothers. Nutrients 15:N/A (2023). PubMed: 37111074
  • Pesti-Asbóth G  et al. Monitoring physiological processes of fast-growing broilers during the whole life cycle: Changes of redox-homeostasis effected to trassulfuration pathway predicting the development of non-alcoholic fatty liver disease. PLoS One 18:e0290310 (2023). PubMed: 37590293
  • Bay ÖF  et al. A genome-scale metabolic model of parasitic whipworm. Nat Commun 14:6937 (2023). PubMed: 37907472
  • Salama SA  et al. Galangin attenuates cadmium-evoked nephrotoxicity: Targeting nucleotide-binding domain-like receptor pyrin domain containing 3 inflammasome, nuclear factor erythroid 2-related factor 2, and nuclear factor kappa B signaling. J Biochem Mol Toxicol 36:e23059 (2022). PubMed: 35384154
View all Publications for this product

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1-3 of 3 Abreviews or Q&A

Question

Inquiry: Hello, I am considering ordering Glutathione Reductase (GR) Assay Kit (ab83461) to study this enzyme activity. I have frozen rabbit placenta. Can you advise me of the details / protocol of preparing this tissue for this analysis (ie tissue extract), so that I can confirm my lab has the ability to do this. Do you have knowledge whether this has been done before in this type of tissue and do you anticipate any difficulties? Many thanks,

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Answer

Thank you for contacting us.

The procedure is given in protocol booklet.

https://www.abcam.com/ps/products/83/ab83461/documents/ab83461%20Glutathione%20Reductase%20GR%20Assay%20Kit%20(Website).pdf

Please thaw frozen tissue on ice. Once thawed add assay buffer and proceed for homogenization.

- start with 10-100 mg of tissue
- add 500-1000 ul (406 volume) of assay buffer homogenize using dounce homogenizer 10-50 passes on ice
- If lysis is confirmed spin down and collect the supernatant as required.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Thank you for your reply.

I wish to detect the presence of antioxidant enzymes in the tissue (glutathione peroxidase and reductase, SOD and catalase most important) but also the quantification or activity of each enzyme is important. Essentially I will be comparing tissue from two groups (disease and no disease) and then examining the effect of two drugs:

The total study will be (n = about 6 to 8 tissues per group):

Disease (n=8), No disease (n=8)
Drug A: Group 1 disease (n=8), Group 2 no disease (n=8)
Drug B: Group 3 disease (n=8), Group 4 no disease (n=8)
Drug A + B: Group 5 disease (n=8), Group 6 no disease (n=8)

I appreciate your advice

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Answer

Thank you for contacting us.

Are you planning to use antibodies for detection of these enzyme. Which application you would be interested in e.g. Immunohistochemistry or western blotting. If you are planning to use antibodies then please use are website there are range of antibodies available as per your requirements.

The following kits I can recommend for assaying the activity of these enzyme;

https://www.abcam.com/glutathione-reductase-gr-assay-kit-ab83461.html

https://www.abcam.com/glutathione-peroxidase-assay-kit-ab102530.html

https://www.abcam.com/superoxide-dismutase-activity-colorimetric-assay-kit-ab65354.html

https://www.abcam.com/catalase-assay-kit-ab83464.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question

I am inquiring about your total antioxidant capacity assay kit or similar.

I have frozen placental tissue from rabbit experiments and I am wishing to examine markers of oxidative stress.
The most important markers for me are glutathione peroxidase and reductase, superoxide dismutase (SOD) and catalase.

Can you kindly advise me if any of your products are potentially of use and a quote for cost please. I am in Belgium.

Regards,

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Answer

Thank you for contacting us.

Could you please clarify; do you want to detect these markers in your tissue samples or you just want to assay the activity of these enzymes?

I will be looking forward to hearing from you soon.

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