Glutathione Assay Kit (Fluorometric) (ab65322)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
製品の概要
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製品名
Glutathione Assay Kit (Fluorometric)
Glutathione キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
アッセイタイプ
Quantitative -
全工程の試験時間
2h 00m -
種交差性
交差種: Mammals, Other species -
製品の概要
Glutathione Assay Kit (Fluorometric) (ab65322) provides a simple in vitro assay for detection of total glutathione changes during cellular response to toxicity, apoptosis and other conditions. The assay uses the dye monochlorobimane (MCB), which forms an adduct with glutathione in a reaction catalyzed by glutathione-S-Transferase (GST). The unbound MCB is almost nonfluorescent, whereas it emits a fluorescent blue light (Ex/Em = 380nm/461nm) when bound to reduced or oxidized glutathione. Thus, the amount of glutathione can be easily detected using a fluorometer or a 96-well fluorometric plate reader.
Visit our FAQs page for tips and troubleshooting.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K251 Glutathione Fluorometric Assay Kit. K251-100 is the same size as the 100 test size of ab65322.
Glutathione (GSH) is a tripeptide that contains L-cysteine, L-glutamic acid and glycine. It is the smallest intracellular protein thiol molecule in the cells, which prevents cell damage caused by reactive oxygen species such as free radicals and peroxides. Glutathione exists in reduced (GSH) and oxidized (GSSG) states. Reduced glutathione (GSH) is a major tissue antioxidant that provides reducing equivalents for the glutathione peroxidase (GPx) catalyzed reduction of lipid hydroperoxides to their corresponding alcohols and hydrogen peroxide to water. In the GPx catalyzed reaction, the formation of a disulfide bond between two GSH molecules generates oxidized glutathione (GSSG). The enzyme glutathione reductase (GR) recycles GSSG to GSH with the simultaneous oxidation of β-nicotinamide adenine dinucleotide phosphate (β-NADPH2). In healthy cells, more than 90% of the total glutathione pool is in the reduced form (GSH). When cells are exposed to increased levels of oxidative stress, GSSG accumulates and the ratio of GSSG to GSH increases. An increased ratio of GSSG-to-GSH is an indication of oxidative stress. The monitoring of reduced and oxidized GSH in biological samples is essential for evaluating the redox and detoxification status of the cells and tissues against oxidative and free radicals mediated cell injury.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests GSH Cell Lysis Buffer 1 x 25ml GSH Standard 1 vial GST Enzyme Mix 1 x 200µl MCB Probe 1 x 200µl -
研究分野
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関連性
Glutathione is a small peptide composed of three amino acids: cysteine, glutamic acid, and glycine and is present in tissues in concentrations as high as one millimolar. Glutathione is the principal intracellular low-molecular-weight thiol that plays a critical role in the cellular defense against oxidative and nitrosative stress in mammalian cells. Diminished glutathione levels have been observed in the early stages of apoptosis. -
別名
- GSH
- GSSG
関連製品
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Related Products
画像
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Glutathione Detection Kit (Fluorometric) (ab65322)Dasgupta A et al., PLoS One, 5, , 2010 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Glutathione pool measured in THP-1 macrophages: uninfected cells; WT: infected with M.tuberculosis wild type; KO: infected with M.tuberculosis OppD knock-out; COM: infected with M.tuberculosis OppD knock-out complemented with OppDA gene. 106 cells were infected and lysed by treating them with 100µl of ice cold lysis buffer. Cell lysate was diluted and mixed as described in the kit protocol. After 30 min incubation at 37C, fluorescence was measured at Ex=380nm/ Em=460nm. Results represent the means of ± S.D. of three determinations.
Image obtained from Dasgupta A. et al; PLoS One; 2010 Aug 17; 5(8): e12225. -
Functional Studies - Glutathione Detection Kit (Fluorometric) (ab65322)Glutathione assays were performed using various amounts of Glutathione as indicated. Results were analyzed according to the kit instructions.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (35)
ab65322 は 35 報の論文で使用されています。
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- Tan H et al. Glabridin, a bioactive component of licorice, ameliorates diabetic nephropathy by regulating ferroptosis and the VEGF/Akt/ERK pathways. Mol Med 28:58 (2022). PubMed: 35596156
- Hossain MM et al. Deltamethrin-Evoked ER Stress Promotes Neuroinflammation in the Adult Mouse Hippocampus. Cells 11:N/A (2022). PubMed: 35741090
- Abu Helal R et al. Aortic Fibrosis in Insulin-Sensitive Mice with Endothelial Cell-Specific Deletion of Ceacam1 Gene. Int J Mol Sci 23:N/A (2022). PubMed: 35457157
- Que R et al. Decursin ameliorates carbon-tetrachloride-induced liver fibrosis by facilitating ferroptosis of hepatic stellate cells. Biochem Cell Biol 100:378-386 (2022). PubMed: 35785548