AB284572

Glutamine Synthetase Activity Assay Kit (Colorimetric)

Name: Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572) | Abcam
Brand: Abcam Limited
SKU: AB284572
Price: 86000 JPY
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(2 Publications)

Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572) is a simple, plate-based colorimetric assay for measuring Glutamine Synthetase activity in biological samples.

別名を表示する

Glutamine-dependent NAD(+) synthetase, NAD(+) synthase [glutamine-hydrolyzing], NAD(+) synthetase, NADSYN1, GLNS, GLUL, Glutamine synthetase, GS, Glutamate--ammonia ligase, Palmitoyltransferase GLUL

4 Images

Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)

FuncS

Unknown

Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)

Glutamine Sythetase Specific Activity in Rat Brain Extracts.

Glutamine Synthetase specific activity in rat brain (3.3 μg), rat liver (7.2 μg) and 3T3 cell lysates (14 μg). All assays were performed following kit protocols.

FuncS

Unknown

Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)

Enzyme kinetics of rat brain extracts (3.3 μg).

FuncS

Unknown

Functional Studies - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)

ADP Standard Curve Example

Other - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)

Other

Supplier Data

Other - Glutamine Synthetase Activity Assay Kit (Colorimetric) (AB284572)

Representative image of Glutamine Synthetase Activity Assay Kit (Colorimetric) ab284572

Components shown from left to right :

- OxiRed Probe

- Converter Mix II

- ADP Standard

- Developer Mix A

- ATP V (2 vials)

- Glutamate

- GS Assay Buffer I

- Glutamine Synthetase

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

検出方法

Colorimetric

サンプルタイプ

Tissue Lysate, Cell Lysate

アッセイタイプ

Enzyme activity

検出感度

>= 10 µU

アッセイプラットフォーム

Microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

製品の詳細

Glutamine Synthetase Activity Assay Kit (Colorimetric) (ab284572) is a simple, plate-based colorimetric assay for measuring Glutamine Synthetase activity in biological samples.

How the assay works
In the Glutamine Synthetase assay, Glutamine Synthetase hydrolyzes Glutamate to Glutamine and ADP. ADP in the subsequent enzymatic reaction, in presence of ADP Converter, ADP Developer Mix and ADP Probe forms a colorimetric product that is measured at OD 570 nm.

Glutamine Synthetase assay protocol summary
- Add samples and standards to wells
- Add reaction mix and incubate
- Analyze with microplate reader at 570 nm in kinetic mode for 60 min at 37°C

Other Notes
This product was previously called K2056 Biovision Glutamine Synthetase Activity Assay Kit (Colorimetric). Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

style

left-column

製品内容

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab284572-100Test", "assetComponentDetails": [ { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB284572-CMP03", "productcode":"AB309437" }, { "size":"1 x 1.2 mL", "name":"Glutamate", "number":"AB284572-CMP07", "productcode":"" }, { "size":"1 x 35 mL", "name":"GS Assay Buffer I", "number":"AB284572-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"ATP V", "number":"AB284572-CMP05", "productcode":"" }, { "size":"1 x 1 Vial", "name":"ADP Standard", "number":"AB284572-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Converter Mix II", "number":"AB284572-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Glutamine Synthetase", "number":"AB284572-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Mix A", "number":"AB284572-CMP02", "productcode":"AB309440" } ] } } }

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Glutamine synthetase also known as glutamine s synthetase or glnA is an enzyme that catalyzes the ATP-dependent conversion of glutamate and ammonia into glutamine. This reaction plays an important role in nitrogen metabolism. Glutamine synthetase has a molecular weight of approximately 620 kDa and forms a multimeric structure commonly seen in bacteria plants and animal tissues with significant expression in the brain liver and kidneys.

Biological function summary

This enzyme supports the detoxification of ammonia by incorporating it into glutamine an essential amino acid and nitrogen donor. Glutamine synthetase operates independently rather than as part of a larger protein complex. It assists in maintaining cellular nitrogen balance and facilitates the synthesis of proteins and other nitrogen-containing molecules. Glutamine peptides serve vital roles in cellular processes underlining the significance of their synthesis.

Pathways

Glutamine synthetase integrates into the glutamate and glutamine cycle between neurons and glial cells highlighting its part in neurotransmitter metabolism. It also features prominently in the urea cycle influencing nitrogen disposal in organisms. Glutamine synthetase interacts with glutaminase which assists in transforming glutamine back to glutamate maintaining a balance of nitrogenous compounds within these pathways.

Glutamine synthetase abnormalities link to hepatic encephalopathy and neurodegenerative disorders such as Alzheimer's disease. Altered enzyme expression contributes to increased ammonia levels adversely affecting brain function. In Alzheimer's disease connections with tau and amyloid-beta proteins suggest a link between glutamine synthetase activity and neurotoxic events. Understanding these interactions may offer insights into therapeutic approaches for these conditions.

style

left-column

製品プロトコール

style

left-column

ターゲットの情報

Catalyzes the final step of the nicotinamide adenine dinucleotide (NAD) de novo synthesis pathway, the ATP-dependent amidation of deamido-NAD using L-glutamine as a nitrogen source.

See full target information NADSYN1

追加のターゲット

GLUL

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of human genetics 111:729-741 PubMed38579670

2024

Clustered de novo start-loss variants in GLUL result in a developmental and epileptic encephalopathy via stabilization of glutamine synthetase.

Applications

Unspecified application

Species

Unspecified reactive species

Amy G Jones,Matilde Aquilino,Rory J Tinker,Laura Duncan,Zandra Jenkins,Gemma L Carvill,Stephanie J DeWard,Dorothy K Grange,M J Hajianpour,Benjamin J Halliday,Muriel Holder-Espinasse,Judit Horvath,Silvia Maitz,Vincenzo Nigro,Manuela Morleo,Victoria Paul,Careni Spencer,Alina I Esterhuizen,Tilman Polster,Alice Spano,Inés Gómez-Lozano,Abhishek Kumar,Gemma Poke,John A Phillips,Hunter R Underhill,Gregory Gimenez,Takashi Namba,Stephen P Robertson

Nature communications 14:6949 PubMed37914686

2023

A carbon-nitrogen negative feedback loop underlies the repeated evolution of cnidarian-Symbiodiniaceae symbioses.

Applications

Unspecified application

Species

Unspecified reactive species

Guoxin Cui,Jianing Mi,Alessandro Moret,Jessica Menzies,Huawen Zhong,Angus Li,Shiou-Han Hung,Salim Al-Babili,Manuel Aranda

View all publications

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Glutamine Synthetase Activity Assay Kit (Colorimetric)