Glutamate Assay Kit (ab83389)

I have contacted the lab and they have confirmed that we can add the Glutamate assay buffer to the catalogue to purchase separately to dilute your samples.

However, the lab have also said that you can simply dilute yoursamples in deionized distilled water and then add to the well. Fill up the volume to 50 ul using the assay buffer. This is obviously the cheaper option but if you would like me to add the assay buffer to the catalogue, please let me know and I will do so.

Unfortunately neither of these kits will be able to detect glutamic acid in peptides/proteins. ab83389 only measures free glutamate levels not glutamic acid and ab138883 is based on enzymatic reactions so it is most likely that it will not react with the glutamate in peptide or proteins.

Non normalement les ions NH3 ne devraient pas interférer le dosage avec ce kit.

The Glutamate Assay Kit (ab83389) can be used to asses the level of glutamate in a range of different biological samples. Unfortunately, the dilution range required will need to be assessed for the different samples you are hoping to use the kit with. This is not something we would be able to predict and I do not have any information of these exact samples having been used. You will need to perform a pilot experiment in order to find out at which dilutions the concentration of glutamate falls within the linear range of the standard curve.

I've checked with the laboratory, and this kit is very specific for glutamate only. Glutamine is not going to cross react and produce false signals. So, no optimization is required and the glutamine media will be fine.

J’ai eu une réponse du laboratoire concernant la préparation de vos échantillons. Les composants de votre tampon ne devraient pas causer d’interférence avec ce kit. Cependant, le laboratoire recommande de diluer vos échantillons dans le tampon qui vient avec le kit puisque il contient des éléments essentiels qui sont nécessaire pour la réaction et qui ne sont pas présent dans votre tampon.

Thank you for contacting us.

After doing some research on this subject it seems that astrocytes release glutamate in a Ca2+ dependent manner. It is a very well-known function/property of astrocytes involving transmitter uptake and release.

Here is the wiki page explaining this phenomenon: http://en.wikipedia.org/wiki/Astrocyte

I also add the link to a couple of papers explaining this phenomenon:

http://www.pnas.org/content/97/15/8629

http://www.sciencedirect.com/science/article/pii/S0197018603002900

The kit (ab83389) is very specific in detecting only glutamate since it utilizes a glutamate specific substrate.

You may need to change certain conditions in the cell media and play around with the incubation times; some glutamate uptake as well should also be seen.

I hope this helps. Please do not hesitate to contact us for further assistance.

Thank you for contacting us.
Though these kits has not been tested with rat samples however based on the material used in these kits these will compatible with all type of mammalian cells.
I can confirm that these kits will be suitable for Rat samples as well.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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