Glucose Assay Kit (ab65333)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 1 µM
製品の概要
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製品名
Glucose Assay Kit
Glucose キット 製品一覧 -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate -
アッセイタイプ
Quantitative -
検出感度
1 µM -
検出範囲
1 µM - 10000 µM -
全工程の試験時間
0h 40m -
製品の概要
Glucose Assay Kit ab65333 is a rapid, simple and sensitive assay used to quantify glucose levels in biological samples such as serum, plasma, and other body fluids, food, growth medium, etc.
How the assay works
In the glucose assay protocol, the glucose enzyme mix oxidizes glucose to generate a product which reacts with a dye to generate color (λ = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportionally to the amount of glucose.
Glucose assay protocol summary
- Add samples (deproteinized) and standards to wells.
- Add reaction mix and incubate for 30 min at 37°C.
- Analyze with microplate reader.
Related Glucose assay products
If you have reducing substances in your samples, we recommend using Glucose Assay Kit - reducing agent compatible ab102517.
For glucose uptake assays, we provide:
- 2-deoxyglucose-based colorimetric glucose uptake assay ab136955, and fluorometric glucose uptake assay ab136956. 2-deoxyglucose is metabolized to 2-DG-6-phosphate, which accumulates within cells and can be quantified with an enzymatic assay.
- We also provide 2-NBDG Glucose Uptake Assay Kit ab287845 and solid chemical 2-NBDG ab146200. 2-NBDG is a fluorescent glucose analog that accumulates in cells.
Related and recommended products
Review our metabolism assays overview to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress here.
Glucose assay methods
There are 3 main enzymatic glucose assay methods, based on different enzymes that act on glucose.
- a) Glucose oxidase-based assays, such as ab65333, that rely on the production of hydrogen peroxide by glucose oxidase. This is then followed by the oxidation of a substrate by a peroxidase using the hydrogen peroxide to produce a colorimetric or fluorometric readout. This method is also referred to as the GOD-POD glucose assay method (GOD-POD = glucose oxidase-peroxidase).
- b) Glucose dehydrogenase-based assays, such as ab102517, that rely on the production of NADH from NAD as part of the action of glucose dehydrogenase on glucose. The increase in NADH can be measured by absorbance at 340nm, or indirectly using the reduction of a tetrazolium dye by NADH to produce a colored or fluorescent product.
- c) Hexokinase-based assays, rely on the production of glucose-6-phosphate by hexokinase from glucose and ATP. Glucose-6-phosphate dehydrogenase then acts on glucose-6-phosphate and NAD or NADP to produce NADH or NADPH, which can be measured in the same way as in glucose dehydrogenase-based assays.
Older glucose assay methods, that are now less commonly used, include:
- Reducing methods, relying on the ability of glucose to reduce a metal ion when glucose is oxidized. This method is non-specific and any strong reducing agent present in the sample will result in an increased signal.
- Condensation with o-toluidine, where the aldehyde group of glucose reacts with o-toluidine to form a glucosamine with a green color. Mannose and galactose tend to cross-react with o-toluidine, however these are found in limited quantities in many sample types. The major disadvantage of this assay is that o-toluidine is corrosive and toxic.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K606 Glucose Assay Kit. -
特記事項
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2000 tests Assay Buffer II 1 x 25ml 20 x 25ml Development Enzyme Mix II 1 vial 20 vials OxiRed Probe 1 x 200µl 20 x 200µl Glucose Standard 1 x 100µl 20 x 100µl -
研究分野
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関連性
Glucose (C6H12O6; FW: 180.16) is a ubiquitous energy source in most organisms, from bacteria to humans. The breakdown of carbohydrates produces mono- and disaccharides, most of which is glucose. Through glycolysis and TCA (citric acid cycle), glucose is oxidized to eventually form CO2 and water, generating the universal energy molecule ATP. Glucose is a primary source of energy for the brain and a critical component in the production of proteins and in lipid metabolism and therefore measurement of glucose level is a key diagnostic parameter for many metabolic disorders.
画像
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Functional studies- ab65333Image from Shao W et al., PLoS One 7(1), Fig 2C. Doi: 10.1371/journal.pone.0028784. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Shao et al investigated the functional outcome of long- term curcumin supplementation on glucose homeostasis. Glucose metabolism was determined in animals with low fat diet (LFD), high fat diet (HFD) and HFD with curcumin feeding using ab65333. Intraperitoneal insulin tolerance tests(IPITT) were conducted at the end of the 26 weeks. It was concluded curcumin improves insulin sensitivity and disposal of glucose.
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Functional Studies - Glucose Assay Kit (ab65333)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Glucose Assay Kit (ab65333)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Functional Studies - Glucose Assay Kit (ab65333)Glucose measured in cell lysates showing quantity (nmol) per million cells.
Samples with the concentration of 2x107 cells/mL were used. Samples were diluted 1.5-13.5 fold and measured colorimetrically.
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Functional Studies - Glucose Assay Kit (ab65333)Glucose measured in human biological fluids showing quantity (µmol) per mL of tested sample. Samples were diluted 13.5 fold and measured colorimetrically.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (219)
ab65333 は 219 報の論文で使用されています。
- Bamgbose G & Tulin A PARP-1 is a transcriptional rheostat of metabolic and bivalent genes during development. Life Sci Alliance 7:N/A (2024). PubMed: 38012002
- Fertan E et al. Cerebral organoids with chromosome 21 trisomy secrete Alzheimer's disease-related soluble aggregates detectable by single-molecule-fluorescence and super-resolution microscopy. Mol Psychiatry 29:369-386 (2024). PubMed: 38102482
- Verissimo T et al. PCK1 is a key regulator of metabolic and mitochondrial functions in renal tubular cells. Am J Physiol Renal Physiol 324:F532-F543 (2023). PubMed: 37102687
- Peritore-Galve FC et al. Increased intestinal permeability and downregulation of absorptive ion transporters Nhe3, Dra, and Sglt1 contribute to diarrhea during Clostridioides difficile infection. Gut Microbes 15:2225841 (2023). PubMed: 37350393
- Huang B et al. Knockdown of circADAM9 inhibits cell progression and glycolysis by targeting the miR-1236-3p/FGF7 axis in breast cancer. Thorac Cancer 14:2350-2360 (2023). PubMed: 37385973