Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Urine, Whole Blood
- Sensitivity: 0.3 mIU/ml
製品名Glucose 6 Phosphate Dehydrogenase Activity Assay Kit (Fluorometric)
Glucose 6 Phosphate Dehydrogenase キット 製品一覧
サンプルの種類Urine, Serum, Plasma, Other biological fluids, Whole Blood, Cell Lysate
アッセイタイプEnzyme activity (quantitative)
検出感度>= 0.3 mIU/ml
種交差性交差種: Mammals, Other species
Glucose 6 Phosphate Dehydrogenase Activity Assay Kit (Fluorometric) (ab176722) provides a simple method for detecting glucose 6 phosphate dehydrogenase (G6PD) activity in a variety of biological samples such as serum, plasma and urine, as well as cell extracts. In the assay, G6PD present in the sample converts NADP+ to NADPH, which can be specifically monitored by a fluorogenic NADPH sensor to yield a highly red fluorescence product. The signal can be easily detected at Ex/Em = 540/590 nm in a fluorescent microplate reader. The fluorescence intensity is proportional to the G6PD activity present in the sample.
The assay can detect glucose 6 phosphate dehydrogenase activity from as low as 0.3 mU/mL in a 100 µL reaction volume.
Glucose 6 Phosphate Dehydrogenase (G6PD, G6PDH, EC 126.96.36.199) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway (PPP). It is a critical metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The production of NADPH is of great importance for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. The NADPH also maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Deficiencies in G6PD predispose individuals to non-immune hemolytic anemia.
保存方法Store at -20°C. Please refer to protocols.
内容 200 tests Assay Buffer 1 x 10ml Enzyme Probe 2 vials G6PD Standard 1 vial NADP 1 vial
関連性Glucose 6 phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands.
G6PD was measured with Abcam's Glucose 6 Phosphate Dehydrogenase Assay Kit (Fluorometric) (ab176722) in a 96-well black plate using a microplate reader. As low as 0.3 mU/mL Glucose 6 Phosphate Dehydrogenase in 100 µL volume can be detected with 1 hour incubation.
The concentration of the Glucose-6-Phosphate Dehydrogenase in X-axis is based on the 50 uL standard, the final in-well concentration should be 1/2 for each point.
ab176722 は 5 報の論文で使用されています。
- Lu F et al. Thioredoxin 1 supports colorectal cancer cell survival and promotes migration and invasion under glucose deprivation through interaction with G6PD. Int J Biol Sci 18:5539-5553 (2022). PubMed: 36147458
- Varghese MV et al. Glucose-6-phosphate dehydrogenase deficiency contributes to metabolic abnormality and pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol 320:L508-L521 (2021). PubMed: 33502933
- Zhang Y et al. Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation. Cell Metab 33:94-109.e8 (2021). PubMed: 33159852
- Nakamura M et al. Glucose-6-phosphate dehydrogenase correlates with tumor immune activity and programmed death ligand-1 expression in Merkel cell carcinoma. J Immunother Cancer 8:N/A (2020). PubMed: 33361404
- Kurdyukov S et al. New cases of Glucose-6-Phosphate Dehydrogenase deficiency in Pulmonary Arterial Hypertension. PLoS One 13:e0203493 (2018). PubMed: 30161219