AB83382

Galactose Assay Kit

Name: Galactose Assay Kit (ab83382) | Abcam
Brand: Abcam Limited
SKU: AB83382
Price: 85000 JPY
Availability: InStock

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(5 Publications)

6 Images

Functional Studies - Galactose Assay Kit (AB83382)

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Lab

Functional Studies - Galactose Assay Kit (AB83382)

Galactose levels measured in biological fluids showing quantity (nmol) per mL. Samples tested undiluted.

FuncS

Lab

Functional Studies - Galactose Assay Kit (AB83382)

Galactose measured in tissue lysates showing quantity (nmol) per mg of extracted protein.

Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were tested undiluted.

FuncS

Lab

Functional Studies - Galactose Assay Kit (AB83382)

Galactose measured in cell lysates showing quantity (nmol) per 1 mln cells.

Samples with the concentration of 4e6 cells/mL were used. Samples were tested undiluted.

FuncS

Supplier Data

Functional Studies - Galactose Assay Kit (AB83382)

Galactose Standard Curve – Fluorometric mode. Example of Fluorometric galactose standard curve obtained with ab83382 in fluorometric mode.

FuncS

Supplier Data

Functional Studies - Galactose Assay Kit (AB83382)

Galactose Standard Curve – Colorimetric mode. Example of galactose standard curve obtained with ab83382 in colorimetric mode.

Schematic Diagram - Galactose Assay Kit (AB83382)

Schematic Diagram

Supplier Data

Schematic Diagram - Galactose Assay Kit (AB83382)

Representative image of Galactose Assay Kit ab83382

Components shown from left to right :

- OxiRed Probe

- Galactose Standard

- Developer Solution V

- Galactose Enzyme Mix

- Assay Buffer 2

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

検出方法

Colorimetric/Fluorometric

サンプルタイプ

Urine, Plasma, Tissue Extracts, Cell culture supernatant, Serum, Other biological fluids

アッセイタイプ

Quantitative

検出感度

< 0.007 nmol/well

アッセイ時間

40m

アッセイプラットフォーム

Microplate reader

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製品の詳細

Galactose Assay Kit (Colorimetric/Fluorometric) (ab83382) provides a simple and convenient method for direct measurement of galactose levels in various biological samples such as serum, plasma and other body fluids, as well as food and growth media. In this assay, oxidation of galactose generates a product that reacts with the galactose probe to produce color (OD570nm) or fluorescence (Ex/Em 535/587 nm). Liquid samples can be tested directly without purification. The assay is fast, convenient and sensitive. It can also be used as a high throughput assay.

High concentrations of lactose could result in elevated readings for free galactose. Galactose oxidase is not completely selective, and it can react with the galactose moiety of lactose.

Visit our FAQs page for tips and troubleshooting.

This product is manufactured by BioVision, an Abcam company and was previously called K621 Galactose Colorimetric/Fluorometric Assay Kit. K621-100 is the same size as the 100 test size of ab83382.

Galactose (C₆H₁₂O₆ MW: 180.16 g/mol) is one of the major naturally occurring sugars, a monosaccharide that differs from glucose only by the configuration of the hydroxyl group at the carbon-4 position. Often present as an anomeric mixture of α-D-galactose and β-D-galactose, this monosaccharide exists abundantly in milk, dairy products and many other food types such as fruits and vegetables.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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製品内容

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab83382-100Test", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Galactose Enzyme Mix", "number":"AB83382-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Developer Solution V", "number":"AB83382-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 2", "number":"AB83382-CMP02", "productcode":"" }, { "size":"1 x 0.2 mL", "name":"OxiRed™ Probe", "number":"AB83382-CMP04", "productcode":"" }, { "size":"1 x 100 µL", "name":"Galactose Standard", "number":"AB83382-CMP05", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Galactose a simple sugar with a molecular weight of 180.16 g/mol is a monosaccharide similar to glucose. It serves as an energy source and forms part of glycoproteins and glycolipids. In the human body the liver ovaries and seminal vesicles express galactose. Cells in these tissues utilize galactose for various cellular activities contributing to functions like cell-cell recognition and signaling. Common assays measure galactose concentrations to understand metabolic conditions and tools like galactose assay kits offer specific insights.

Biological function summary

Galactose plays a critical role in the Leloir pathway converting galactose into glucose-1-phosphate. This conversion involves several enzymes including galactokinase and uridylyltransferase. Not existing within any larger complex galactose activity supports the proper metabolism of carbohydrates and the synthesis of complex biomolecules. Its function ensures proper cellular metabolism by providing precursors for energy production and biosynthesis.

Pathways

Galactose contributes significantly to the glycolysis and gluconeogenesis processes. UDP-galactose an activated form of galactose interacts in these metabolic pathways highlighting its role in carbohydrate metabolism. The protein galactokinase is essential in phosphorylating galactose for its subsequent incorporation into these pathways. Another protein galactose-1-phosphate uridylyltransferase further catalyzes the transformation of galactose derivatives showing the interconnectedness of these proteins within metabolic networks.

Defective galactose metabolism leads to conditions like galactosemia a genetic disorder affecting an enzyme in the Leloir pathway. Classical galactosemia involves mutations in the galactose-1-phosphate uridylyltransferase gene causing an accumulation of galactose-1-phosphate in tissues and organs leading to toxicity. A related condition congenital cataracts can arise from galactose pathway disruptions where crystallins proteins in the lens of the eye are affected by elevated galactitol resulting from excess galactose. Understanding galactose's biological role helps monitor and manage these disorders effectively.

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製品プロトコール

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ターゲットの情報

See full target information D-Galactose

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Clinical pharmacokinetics 61:1039-1055 PubMed35570253

2022

Liver Cirrhosis Affects the Pharmacokinetics of the Six Substrates of the Basel Phenotyping Cocktail Differently.

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Urs Duthaler,Fabio Bachmann,Claudia Suenderhauf,Tanja Grandinetti,Florian Pfefferkorn,Manuel Haschke,Petr Hruz,Jamal Bouitbir,Stephan Krähenbühl

Journal of nanobiotechnology 19:363 PubMed34789275

2021

A nanodrug system overexpressed circRNA_0001805 alleviates nonalcoholic fatty liver disease via miR-106a-5p/miR-320a and ABCA1/CPT1 axis.

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Jian Li,Jing Qi,Yishu Tang,Huaizheng Liu,Kefu Zhou,Zheren Dai,Lehong Yuan,Chuanzheng Sun

ACS synthetic biology 9:508-516 PubMed32119773

2020

Yeast Surface Display System for Facilitated Production and Application of Phage Endolysin.

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Jihwan Chun,Jaewoo Bai,Sangryeol Ryu

Physiological genomics 52:203-216 PubMed32146873

2020

Hepatic proteome analysis reveals altered mitochondrial metabolism and suppressed acyl-CoA synthetase-1 in colon-26 tumor-induced cachexia.

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Andy V Khamoui,Dorota Tokmina-Roszyk,Harry B Rossiter,Gregg B Fields,Nishant P Visavadiya

Animals : an open access journal from MDPI 6: PubMed27792138

2016

Intermittent Suckling in Combination with an Older Weaning Age Improves Growth, Feed Intake and Aspects of Gastrointestinal Tract Carbohydrate Absorption in Pigs after Weaning.

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Diana L Turpin,Pieter Langendijk,Tai-Yuan Chen,John R Pluske

View all publications

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Galactose Assay Kit