AB325574

Fibronectin Cell Adhesion Assay (Fluorometric)

Name: Fibronectin Cell Adhesion Assay (Fluorometric) (ab325574) | Abcam
Brand: Abcam Limited
SKU: AB325574
Price: 120000 JPY
Availability: InStock

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ab325574 Fibronectin Cell Adhesion Assay (Fluorometric) kit provides a rapid, quantitative method for evaluating cell adhesion. The kit contains sufficient reagents for the evaluation of 48 samples (40 Human Fibronectin-coated wells, 8 BSA-coated wells).

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Functional Studies - Fibronectin Cell Adhesion Assay (Fluorometric) (AB325574)

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Supplier Data

Functional Studies - Fibronectin Cell Adhesion Assay (Fluorometric) (AB325574)

ECM-mediated Cell Adhesion. Serum starved cells were allowed to attach to ECM-coated 48-well plate for 1 hr at 100,000 cells/well. Adherent cells were lysed and quantified by GR Dye as described in Assay Protocol.

This figure demonstrates typical results with ab325574 Fibronectin Cell Adhesion Assay (Fluorometric). One should use the data below for reference only. This data should not be used to interpret actual results.

FuncS

Supplier Data

Functional Studies - Fibronectin Cell Adhesion Assay (Fluorometric) (AB325574)

Quantitation of Human HT-1080. HT-1080 cells were titrated in 1X PBS, then subsequently lysed and detected with 1X Lysis Buffer/GR Dye (225 µL of cell suspension was mixed with 75 µL of 4X Lysis Buffer and 1 µL of GR dye).

Key facts

検出方法

Fluorometric

アッセイタイプ

Cell-based

Quantitative

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS (Act)": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Cell adhesion is a complex process involved in embryogenesis, migration/invasion, tissue remodeling, and wound healing. To perform these processes, cells adhere to extracellular matrix components (via adhesion receptors), forming complexes with components of the cytoskeleton that ultimately affect cell motility, differentiation, proliferation, and survival.

ab325574 Fibronectin Cell Adhesion Assay (Fluorometric) kit utilizes a Fibronectin-coated 48-well plate. First, cells are seeded onto the coated substrate, where the adherent cells are captured. Next, unbound cells are removed with consecutive washes. Finally, the adherent cells are lysed and subsequently detected with GR Dye.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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製品内容

{ "values": { "48Tests": { "sellingSize": "48 Tests", "publicAssetCode":"ab325574-48Tests", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"4X Lysis Solution", "number":"AB325574-CMP02", "productcode":"" }, { "size":"1 x 50 µL", "name":"GR Dye", "number":"AB325574-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Fibronectin Adhesion Plate", "number":"AB325574-CMP01", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Ambient - Cannot Ship with Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

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websiteProtocolBooklet

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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Fibronectin Cell Adhesion Assay (Fluorometric)