AB241041

Factor XII / XIIa Assay Kit

Name: Factor XII / XIIa Assay Kit (ab241041) | Abcam
Brand: Abcam Limited
SKU: AB241041
Price: 129000 JPY
Availability: InStock

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Factor XIIa Assay Kit ab241041 uses the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm).

別名を表示する

Coagulation factor XII, Hageman factor, HAF, F12

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Functional Studies - Factor XII / XIIa Assay Kit (AB241041)

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Functional Studies - Factor XII / XIIa Assay Kit (AB241041)

Factor XII is converted to it’s active form (XIIa) by the Coagulation Activator. Factor XIIa cleaves Prekallikrein into active Kallikrein which in turn cleaves the substrate releasing a colorimetric product. In the presence of the inhibitor, Factor XIIa is inactivated (XIIi) by the inhibitor and cannot activate Prekallikrein.

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Functional Studies - Factor XII / XIIa Assay Kit (AB241041)

Kinetic progressive curves for different amounts of FXIIa Enzyme

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Functional Studies - Factor XII / XIIa Assay Kit (AB241041)

Standard curve for pNA.

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Functional Studies - Factor XII / XIIa Assay Kit (AB241041)

Kinetic progressive curves for different amounts of Activated Plasma Samples are shown.

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Functional Studies - Factor XII / XIIa Assay Kit (AB241041)

PK activity. Standard curve for pNA (n = 3) was used to estimate FXIIa activity in Normal Pooled Human Plasma and FXII-deficient Human Plasma (n = 3) (D). Assays were performed according to the kit protocol.

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma

検出感度

= 0.5 mU

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

The Factor XIIa Assay Kit (ab241041) utilizes the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm). The kit is easy-to-use and can detect Factor XIIa (as low as 1 mPEU) from plasma samples. This kit also measures Factor XII. In the sample preparation, the FXII is converted to FXIIa by the FXII activator.

This product is manufactured by BioVision, an Abcam company and was previously called K994 Factor XIIa Activity Assay Kit (Colorimetric). K994-100 is the same size as the 100 test size of ab241041.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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製品内容

{ "values": { "100Test": { "sellingSize": "100 Test", "publicAssetCode":"ab241041-100Test", "assetComponentDetails": [ { "size":"1 x 1 mL", "name":"Coagulation Activator Solution", "number":"AB241041-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"pNA Standard I", "number":"AB241041-CMP07", "productcode":"" }, { "size":"1 x 100 µL", "name":"FXIIa Inhibitor", "number":"AB241041-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Assay Buffer 35", "number":"AB241041-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human Prekallikrein", "number":"AB241041-CMP08", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human Factor XIIa", "number":"AB241041-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"Kallikrein Substrate", "number":"AB241041-CMP04", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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製品プロトコール

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ターゲットの情報

Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa (PubMed : 2019570, PubMed : 21304106, PubMed : 8427954).

See full target information F12

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websiteProtocolBooklet

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Factor XII / XIIa Assay Kit