Factor VII Human Chromogenic Activity Assay Kit (ab108830)

Thank you for your call last week and for your patience. I apologize for the extended delay in getting this information for you.

I've just received a reply from the lab, and these kits can be analyzed at room temperature. The OD might be slightly lower than at 37C but the kinetics should not be affected. If you warm your samples to 37C prior to analysis and then read the plate at room temperature, there should not be a noticeable effect. We would still recommend analyzing the kinetic data as opposed to an end point analysis.

I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you. I'll be happy to help.

Thank you for your call today.

As we discussed, you can email mailto:us.sales@abcam.com for information regarding bulk purchases.

Please let me know if you have any questions or if there is anything else that we can do for you.

Thank you for your reply.

Unfortunately we are unable to run inhibitor tests for our customers.

However, if I can help you in any other way please do letme know.

Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you.


The Factor VII Human Chromogenic Activity Assay Kit had been developed to measure human FVII activity in plasma and cell culture supernatants. The assay works by binding the FVII present in the sample to the plate by a monoclonal antibody pre-coated on the plate. The activity of the FVII is then quantified by introducing a FXa substrate which upon cleavage releases ayellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the FVII enzymatic activity.


The Factor X Human Chromogenic Activity Assay Kit (ab108833) similarlymeasures the activation of zymogen Factor X to Factor Xa by RVV. The amidolytic activity of the Factor Xa isquantified using a highly specific Factor Xa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the Factor X enzymatic activity.


We have had reports of customers using these kits to test inhibitors. We have not performed this ourselves in-house and some optimisationmay therefore be required. It may be that as you want to measure the inhibition of activity of your compounds, not the Factor VII or X activity within a biological sample, for which the kit is designed, you may need more Factor X and VII standard than what the kit provides.


The standard provided with the kit is enough for two sets of duplicate standard curve. If you require more we may be able to sell the FVII and Factor X standard separately.If you would like me to look into this please let me know.


I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Thank you for your enquiry.  I can confirm that this kit can measure both Factor VII and Factor VIIa activity since this kit will activite Factor VII to become Factor VIIa by tissue factor. However, this kit will not be able to differentiate between  Factor VII and Factor VIIa in the results.  Unfortunately, we do not have any kits that can detect only Factor VIIa but I was able to find a different supplier on Antibodies on-line website. http://www.antibodies-online.com/kit/612646/Factor+VIIa+FVIIa+Chromogenic+Activity+Assay/ Also, I have attached a PDF print out of another Factor VIIa kit that is available in Japan. I hope this information will be helpful. If there is anything else that I can help you with please do not hesitate to contact me. Have a nice day!