ECL Western Blotting Substrate Kit (ab65623)
製品の概要
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製品名
ECL Western Blotting Substrate Kit -
製品の概要
The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate offers superior signal and clear background. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.
Our ECL kits include this kit ab65623 and our high sensitivity ECL substrate kits:
- High Sensitivity ECL Substrate Kit ab133406 to detect 23pg-187ng of protein per band
- Very High Sensitivity ECL Substrate Kit ab133408 to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit ab133409 to detect 1.2pg-2ng of protein per band
Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K820 ECL Western Blotting Substrate Kit. K820-500 is the same size as the 500 test size of ab65623.
Reagents provided are sufficient for 400 cm2 of membrane.
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アプリケーション
適用あり: WBmore details
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 50 tests ECL Reagent A 1 x 25ml ECL Reagent B 1 x 25ml -
研究分野
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関連性
ECL (enhanced luminol-based chemiluminescent) detection is a sensitive, nonradioactive method for easy detection of horseradish peroxidase (HRP) on western blots. -
別名
- Enhanced luminol based chemiluminescent
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65623の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
|
WB
Use at an assay dependent dilution. |
画像
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ECL Western Blotting Substrate Kit - 50 Tests (ab65623)ECL Western Blotting Substrate
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (110)
ab65623 は 110 報の論文で使用されています。
- Chen B et al. N6‑methyladenosine‑induced long non‑coding RNA PVT1 regulates the miR‑27b‑3p/BLM axis to promote prostate cancer progression. Int J Oncol 62:N/A (2023). PubMed: 36484368
- Yan J et al. Long non-coding RNA MIR17HG sponges microRNA-21 to upregulate PTEN and regulate homoharringtonine-based chemoresistance of acute myeloid leukemia cells. Oncol Lett 23:24 (2022). PubMed: 34868361
- Lv F et al. Therapeutic exosomal vaccine for enhanced cancer immunotherapy by mediating tumor microenvironment. iScience 25:103639 (2022). PubMed: 35024580
- Jiang J et al. Circular RNA circHIPK3 is downregulated in diabetic cardiomyopathy and overexpression of circHIPK3 suppresses PTEN to protect cardiomyocytes from high glucose-induced cell apoptosis. Bioengineered 13:6272-6279 (2022). PubMed: 35200097
- Jiang L et al. LncRNA KCNQ1OT1-mediated cervical cancer progression by sponging miR-1270 as a ceRNA of LOXL2 through PI3k/Akt pathway. J Obstet Gynaecol Res 48:1001-1010 (2022). PubMed: 35218109