ECL Substrate Kit (High Sensitivity) (ab133406)
製品の概要
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製品名
ECL Substrate Kit (High Sensitivity)
ECL Substrate キット 製品一覧 -
製品の概要
High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with CCD imaging. The high sensitivity ECL substrate produces a strong signal with very low background. Additionally, the ECL signal is long lasting, allowing repeated exposures without fear of losing data.
Our ECL kits include our popular ECL Substrate Kit ab65623 and our high sensitivity ECL substrate kits:
- this kit (High Sensitivity ECL Substrate Kit ab133406) to detect 23pg-187ng of protein per band
- Very High Sensitivity ECL Substrate Kit ab133408 to detect 4.6pg-4.7ng of protein per band
- Ultra High Sensitivity ECL Substrate Kit ab133409 to detect 1.2pg-2ng of protein per band
Detection ranges in pg and ng stated above should be used for guidance only as detection range is dependent on the molecular weight of a protein.
This product was previously called Optiblot ECL Detect Kit (23pg-187ng).
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特記事項
Use:
- 200ml kit for 2000cm2 membrane
- 500ml kit for 5000cm2 membraneThe primary antibody can often be diluted 5 to 10 fold more than usual when using this ECL substrate. A typical primary antibody dilution range using the substrate is 1/5000 - 1/20,000, with a typical secondary antibody dilution range of 1/20,000 - 1/100,000. Some optimisation may be required.
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アプリケーション
適用あり: WBmore details
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 200 ml 20 ml 500 ml Luminol/Enhancer Solution 1 x 100ml 1 x 10ml 1 x 250ml Peroxide Chemiluminescent Detection Reagent 1 x 100ml 1 x 10ml 1 x 250ml -
研究分野
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133406の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. |
画像
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Western blot comparison 1/25000 anti-Transferrin primary antibody, 1/50000 secondary antibody, 20 second exposure
Anti-Transferrin antibody (ab1223) at 1/25000 dilution. Lanes 1-14 Transferrin protein (ab91435), Loading dilution (Lanes 1-8): 12.5, 6.2, 3.1, 1.6, 0.8, 0.3, 0.19µg protein.
Secondary
HRP conjugated polyclonal to Rabbit IgG (ab97080) at 1/50000 developed using Optiblot ECL Detect and Vendor A ECL subtrate.
Exposure time: 20 seconds
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Western Blot 1/25000 primary antibody, 1/5000 secondary antibody, 20 second exposureEach lane contains the following amount of COX2 recombinant protein (ab58868): 1) 100ng 2) 50ng 3) 25ng 4) 12.5ng 5) 6.25ng 6) 3.13ng 7) 1.56ng 8) 780pg 9) 390pg 10) 195pg 11) 98pg. The blot was probed with rabbit anti-COX2 antibody (ab15191) at 1/25000 dilution and with a rabbit secondary (ab97080) at 1/5000 dilution. The blot was developed with 20ml of Optiblot ECL Detect.
Exposure time: 20 seconds
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (58)
ab133406 は 58 報の論文で使用されています。
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- Ling M et al. IL1A regulates the inflammation in gout through the Toll-like receptors pathway. Int J Med Sci 21:188-199 (2024). PubMed: 38164346
- Eymery MC et al. Linking medicinal cannabis to autotaxin-lysophosphatidic acid signaling. Life Sci Alliance 6:N/A (2023). PubMed: 36623871
- Nie XJ et al. GATA3 ameliorates melanocyte injuries in vitiligo through SIRT3-mediated HMGB1 deacetylation. J Dermatol 50:472-484 (2023). PubMed: 36412048
- Zhao H et al. SIRT1/APE1 promotes the viability of gastric cancer cells by inhibiting p53 to suppress ferroptosis. Open Med (Wars) 18:20220620 (2023). PubMed: 36820068