AB156908

DNA demethylase (total) Activity Quantification Ultra Assay Kit

Name: DNA demethylase (total) Activity Quantification Ultra Assay Kit (ab156908)
Brand: Abcam Limited
SKU: AB156908
Price: 114000 JPY
Availability: InStock

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Functional Studies - DNA demethylase (total) Activity Quantification Ultra Assay Kit (AB156908)

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Supplier Data

Functional Studies - DNA demethylase (total) Activity Quantification Ultra Assay Kit (AB156908)

Assay control standard was added into the assay wells at different concentrations and then measured with Abcam's DNA demethylase (total) Activity Quantification Ultra Assay Kit (ab156908).

Key facts

サンプルタイプ

Nuclear Extracts, Cell culture extracts, Tissue Extracts

アッセイ時間

アッセイプラットフォーム

Microplate reader

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Enzyme activity assay": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Abcam's DNA demethylase (total) Activity Quantification Ultra Assay Kit (ab156908) is a complete set of essential components which enables an experimenter to measure total DNA demethylase activity/inhibition. The kit can be used with nuclear extracts from a broad range of species such as mammals and plants, in a variety of forms including, but not limited to, cultured cells and fresh tissues.

DNA demethylation is necessary for the epigenentic reprogramming of genes and is also directly involved in many important disease mechanisms such as tumor progression. Demethylation of DNA can either be passive or active, or a combination of both. Passive DNA demethylation usually takes place on newly synthesized DNA strands via DNMT1 during replication rounds. Active DNA demethylation mainly occurs by the removal of 5-methylcytosine through further modified cytosine bases which have been converted by TET enzyme-mediated oxidation. These oxidation products have been shown to be repaired by TDG, a glycosylase which is involved in base excision repair, or directly converted to cytosine by DNMT3A/ DNMT3B in oxidative states. It is proposed that DNA demethylation could also be initiated by deamination of 5-mC through candidate deaminases including AID and APOBEC1, which convert 5-mC to thymine. The resulting thymine could be repaired by BER initiated by a T-G mismatch glycosylase such as MBD4 or TDG. In addition, the 5-mC base can be directly removed in plants by the DME/ROS1 family of 5-mC DNA glycosylases, resulting in an abasic site that is repaired by the BER process.

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

Multi

長期保存温度

Multi

保管に関する情報

Please refer to protocols

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製品プロトコール

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ターゲットの情報

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental botany 66:5959-69 PubMed26116024

2015

AtROS1 overexpression provides evidence for epigenetic regulation of genes encoding enzymes of flavonoid biosynthesis and antioxidant pathways during salt stress in transgenic tobacco.

Applications

Unspecified application

Species

Unspecified reactive species

Poonam Bharti,Monika Mahajan,Ajay K Vishwakarma,Jyoti Bhardwaj,Sudesh Kumar Yadav

View all publications

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websiteProtocolBooklet

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Abcam product promise

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DNA demethylase (total) Activity Quantification Ultra Assay Kit