AB109800

Complex III Immunocapture Kit

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(5 Publications)

250 µg, 500 µg or 750 µg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 µg, ~50 µg or ~75 µg respectively of Complex III from heart mitochondria.

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Supplier Data

Immunoprecipitation - Complex III Immunocapture Kit (AB109800)

Complex III immunoprecipitation using antibody ab109862/MS301 crosslinked to protein G-agarose beads as product ab109800.

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Key facts

交差種

Mouse, Rat, Cow, Human

アッセイタイプ

Quantitative

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製品の詳細

250 μg, 500 μg or 750 μg monoclonal antibodies irreversibly crosslinked to protein G-agarose beads which can immunocapture ~25 μg, ~50 μg or ~75 μg respectively of Complex III from heart mitochondria.

ab109800 Complex III Immunocapture Kit allows isolation of the ubiquinol-cytochrome c oxidoreductase complex from small amounts of tissue. This facilitates subsequent analysis of assembly state, activity and the extent of post translational modifications including oxidative damage that occur with aging. Uses for ab109800 include research on genetic mitochondrial disease, where complex III may have an additional role in the stabilization of complex I.

Note: The immunocapture protocol for this kit requires Abcam detergent lauryl maltoside (ab109857/MS910).

**Related products**Review the , or the full to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Complex III also known as cytochrome c reductase or cytochrome bc1 complex plays an important role in the mitochondrial electron transport chain. This multiprotein complex weighing approximately 250 kDa facilitates electron transfer from ubiquinol to cytochrome c. Complex III resides in the inner mitochondrial membrane of eukaryotic cells and is important for cellular energy production. Structurally it consists of 11 subunits in mammals with cytochrome b cytochrome c1 and Rieske iron-sulfur protein being the core components.

Biological function summary

Complex III transfers electrons and contributes to proton translocation across the mitochondrial membrane. This process generates the proton gradient needed for ATP synthesis via oxidative phosphorylation often referred to as OXPHOS. As a core component of the mitochondrial respiratory chain Complex III interacts closely with other complexes mainly Complexes I and IV to maintain efficient energy conversion.

Pathways

Complex III is integral to the oxidative phosphorylation pathway and the respiratory electron transport chain. It ensures efficient electron transport working closely with Complexes I II and IV. Cytochrome c acts as an electron carrier between Complex III and Complex IV in this pathway. This coordinated interaction is essential for ATP generation meeting cellular energy demands.

Complex III is linked with mitochondrial disorders and neurodegenerative diseases. Deficiencies or mutations in its components may lead to disorders such as mitochondrial encephalopathy or Leigh syndrome which are associated with impaired energy metabolism. The complex's dysfunction also affects proteins connected to these diseases like ATP synthase due to disrupted energy production. Additionally its role in oxidative stress has implications for conditions like mitotox-related mitochondrial damage.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

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ターゲットの情報

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:3527 PubMed32669538

2020

Rieske iron-sulfur protein induces FKBP12.6/RyR2 complex remodeling and subsequent pulmonary hypertension through NF-κB/cyclin D1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Mei,Yun-Min Zheng,Tengyao Song,Vishal R Yadav,Leroy C Joseph,Lillian Truong,Sharath Kandhi,Margarida M Barroso,Hiroshi Takeshima,Marc A Judson,Yong-Xiao Wang

Molecular and cellular biology 35:1139-56 PubMed25605331

2015

C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Marjorie Desmurs,Michelangelo Foti,Etienne Raemy,Frédéric Maxime Vaz,Jean-Claude Martinou,Amos Bairoch,Lydie Lane

Free radical biology & medicine 50:945-52 PubMed21238580

2011

Primary role of mitochondrial Rieske iron-sulfur protein in hypoxic ROS production in pulmonary artery myocytes.

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Unspecified application

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Unspecified reactive species

Amit S Korde,Vishal R Yadav,Yun-Min Zheng,Yong-Xiao Wang

BMC endocrine disorders 9:2 PubMed19166612

2009

Alteration of renal respiratory Complex-III during experimental type-1 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Shankar Munusamy,Hamida Saba,Tanecia Mitchell,Judit K Megyesi,Robert W Brock,Lee Ann Macmillan-Crow

Biochimica et biophysica acta 1762:213-22 PubMed16120479

2005

Proteomic analysis of succinate dehydrogenase and ubiquinol-cytochrome c reductase (Complex II and III) isolated by immunoprecipitation from bovine and mouse heart mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Birgit Schilling,James Murray,Chris B Yoo,Richard H Row,Michael P Cusack,Roderick A Capaldi,Bradford W Gibson

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

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websiteProtocolBooklet

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Complex III Immunocapture Kit