Choline/Acetylcholine Assay Kit (ab65345)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture extracts, Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.01 nmol/well
製品の概要
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製品名
Choline/Acetylcholine Assay Kit -
検出方法
Colorimetric/Fluorometric -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts -
アッセイタイプ
Quantitative -
検出感度
> 0.01 nmol/well -
検出範囲
0.01 nmol/well - 5 nmol/well -
全工程の試験時間
0h 40m -
種交差性
交差種: Mammals, Other species -
製品の概要
Choline/Acetylcholine Assay Kit (ab65345) provides a simple and sensitive means for quantifying Choline and Acetylcholine by either a colorimetric or fluorometric method in samples such as blood, cells, culture media, fermentation media, etc.
In the choline / acetylcholine assay protocol, free choline is oxidized to betaine, via the intermediate betaine aldehyde. The reaction generates products which react with the Choline Probe to generate color (λ= 570 nm), and fluorescence (Ex/Em 535/587 nm).
Acetylcholine can be converted to choline by adding acetylcholinesterase to the reaction to measure total choline (choline + acetyl choline). The amount of acetyl choline is calculated by subtracting choline from total choline.
There is no need for pretreatment or purification of samples. The kit can detect ~10 pmol-5 nmol of choline or acetylcholine.
Cells should be cultured in choline chloride free medium.
Choline / acetylcholine assay protocol summary:
- add samples and standards to wells
- add choline reaction mix with, or without, acetylcholinesterase, and incubate for 30 min at room temp
- analyze with microplate reader -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K615 Choline/Acetylcholine Quantification Colorimetric/Fluorometric Kit. K615-100 is the same size as the 100 test size of ab65345.
If you need to purchase additional Choline Probe for this product, please see ab139505.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2000 tests Acetylcholinesterase Enzyme 1 vial 20 vials Assay Buffer VI 1 x 25ml 20 x 25ml Choline Standard 1 vial 20 vials Enzyme Mix IV 1 vial 20 vials OxiRed Probe 1 x 200µl 20 x 200µl -
研究分野
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関連性
Choline and acetylcholine (often abbreviated ACh) play important roles in many biological processes. Choline is a natural amine, and is generally found in phosphatidylcholine and sphingomyelin, phopholipids abundant in cell membranes. Choline is also the precursor molecule for the neurotransmitter acetylcholine which is involved in many functions both in the peripheral nervous system (PNS) and in the central nervous system (CNS). In the PNS, ACh activates muscles and is a major neurotransmitter in the autonomic nervous system. In the CNS, ACh and the associated neurons form the cholinergic system, which tends to cause anti-excitatory actions.
画像
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Functional studies- ab65345Image from Kim J.H et al., PLoS One 8(2), Fig 4. doi: 10.1371/journal.pone.0056736. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Acetylcholine levels from mouse brain tissue was measured using Acetylcholine assay kit (ab65345). Normal mice were anesthetized with thiopental sodium at 20minutes after the end of electroacupuncture (EA) treatment to determine levels of ACh (Figure A). Immunohistochemical staining mAChR (muscarinic acetylcholine receptor) M3 in mouse cerebral cortex tissue 20 minutes after the end of EA treatment (Image B).
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Functional Studies - Choline/Acetylcholine Assay Kit (ab65345) -
Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)Choline measured fluorometrically in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
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Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)Choline measured fluorometrically in cell lysates showing quantity (pmol) per 1 mln of tested cells
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Functional Studies - Choline/Acetylcholine Assay Kit (ab65345)Choline measured fluorometrically in biological fluids showing quantity (nmol) per mL of tested sample
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (62)
ab65345 は 62 報の論文で使用されています。
- Nguyen XTA et al. MFSD7c functions as a transporter of choline at the blood-brain barrier. Cell Res 34:245-257 (2024). PubMed: 38302740
- Jung H et al. Auditory or Audiovisual Stimulation Ameliorates Cognitive Impairment and Neuropathology in ApoE4 Knock-In Mice. Int J Mol Sci 24:N/A (2023). PubMed: 36674449
- Oka N et al. SARS-CoV-2 S1 protein causes brain inflammation by reducing intracerebral acetylcholine production. iScience 26:106954 (2023). PubMed: 37275532
- Munasinghe PE et al. Non-neuronal cholinergic system delays cardiac remodelling in type 1 diabetes. Heliyon 9:e17434 (2023). PubMed: 37426799
- Olatunji AO et al. Effect of daflon-500®, a flavonoid compound on chlorpyriphos-induced oxidative changes in the hypophysis and testes in adult male rats. Toxicol Res 38:345-353 (2022). PubMed: 35874508