Chloride Channel Assay Kit (Colorimetric) (ab176767)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
製品の概要
-
製品名
Chloride Channel Assay Kit (Colorimetric) -
検出方法
Colorimetric -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
製品の概要
Abcam’s Chloride Channel Assay Kit (Colorimetric) (ab176767) provides a sensitive and robust colorimetric method for studying chloride channels. The assay is based on our proprietary iodide indicator (Iodide Sensor Blue dye) to measure iodide concentration, as low as 30 nM of iodide can be detected. Iodide Sensor Blue dye forms a blue complex with iodide, which has absorption spanning from the UV to 700 nm. Thus a few absorption wavelengths can be used for monitoring the iodide-dependent color change.
Abcam's Chloride Channel Assay Kit (Colorimetric) (ab176767) provides an optimized assay method for monitoring chloride channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
-
特記事項
Chloride channels have a variety of important physiological and cellular functions that include regulation of pH, volume homeostasis, organic solute transport, cell migration, cell proliferation and differentiation. Chloride channels represent valuable drug targets. A number of chronic disease states such as cystic fibrosis and Bartter's syndrome are due to defects in chloride channel functions. However, the existing technologies for screening chloride channel modulators are a compromise between throughput, sensitivity and physiological relevance.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate reader
製品の特性
-
保存方法
Store at +4°C. Please refer to protocols. -
内容 10 x 96 tests 100X Iodide Sensor Enhancer 1 x 0.5ml 10X Cell Lysis Buffer 1 x 5ml I-Loading Buffer 1 x 100ml Iodide Sensor Blue dye 1 x 50ml -
研究分野
画像
-
Dose response curve for Chloride Channel Assay Kit (Colorimetric) (ab176767)
Sodium iodide (Nal) dose response was measured with Abcam's Chloride Channel Assay Kit (Colorimetric) (ab176767) in a 96-well black wall/clear bottom plate. As low as 30 nM of Nal was detected with 10 minutes incubation time (n = 3).
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab176767 は 2 報の論文で使用されています。
- Abdul-Salam VB et al. CLIC4/Arf6 Pathway. Circ Res 124:52-65 (2019). PubMed: 30582444
- Venter J et al. Development and functional characterization of extrahepatic cholangiocyte lines from normal rats. Dig Liver Dis 47:964-72 (2015). PubMed: 26277684