Chloride Assay Kit (ab83372)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 20 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.4 mM
製品の概要
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製品名
Chloride Assay Kit -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
アッセイタイプ
Quantitative -
検出感度
> 0.4 mM -
検出範囲
20 nmol/well - 120 nmol/well -
全工程の試験時間
0h 20m -
製品の概要
Abcam's Chloride Assay Kit provides a quick, simple method for quantification of Chloride in a variety of biological samples. Blood and urine can be used directly after dilution with water. The assay is based upon the competition of Hg2+ and Fe2+ for TPTZ. The preferred Hg-TPTZ adduct exhibits no color. In the presence of Chloride, Hg2+ forms HgCl2 freeing up TPTZ which then binds the available Fe2+ giving a very intense absorbance with a λmax~ 620nm. The assay is linear in the range 20 to 120 nmol Chloride/well with detection sensitivity ~0.4 mM chloride.
Visit our FAQs page for tips and troubleshooting. -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K530 Chloride Colorimetric Assay Kit. K530-100 is the same size as the 100 test size of ab83372.
Chloride is the anionic form of chlorine. It is the most common of the anions found in living organisms. Chloride ions play a variety of important physiological roles. Chloride channels are found in a variety of cells and are responsible for setting resting cell membrane potential and regulating cell volume. In the nervous system, the action of glycine and GABA are related to chloride levels in specific neurons. Chloride is also instrumental in maintaining the acid-base balance in blood. The kidneys are instrumental in closely regulating serum chloride levels. There are a number of pathologies associated with defective chloride transport; the most well-known being Cystic Fibrosis, caused by a mutation in CFTR a membrane chloride transporter.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at room temperature. Please refer to protocols. -
内容 100 tests Chloride Reagent 1 x 15ml Chloride Standard 1 vial -
研究分野
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関連性
Chloride is the anionic form of chlorine. It is the most common of the anions found in living organisms. Chloride ions play a variety of important physiological roles. Chloride channels are found in a variety of cells and are responsible for setting resting cell membrane potential and regulating cell volume. In the nervous system, the action of glycine and GABA are related to chloride levels in specific neurons. Chloride is also instrumental in maintaining the acid-base balance in blood. The kidneys are instrumental in closely regulating serum chloride levels. There are a number of pathologies associated with defective chloride transport; the most well-known being Cystic Fibrosis, caused by a mutation in CFTR a membrane chloride transporter.
画像
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Functional Studies - Chloride Assay Kit (ab83372)
Chloride measured in biological fluids. Samples were diluted 3-27 fold.
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Functional Studies - Chloride Assay Kit (ab83372)Chloride Standard Curve: Assays were performed following the kit protocol.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab83372 は 3 報の論文で使用されています。
- Yang HS et al. Respiratory Safety Evaluation in Mice and Inhibition of Adenoviral Amplification in Human Bronchial Endothelial Cells Using a Novel Type of Chlorine Dioxide Gas Reactor. Toxics 10:N/A (2022). PubMed: 35051080
- Shu T et al. Depletion of Tissue-Specific Ion Transporters Causes Differential Expression of PRL Targets in Response to Increased Levels of Endogenous PRL. Front Endocrinol (Lausanne) 9:683 (2018). PubMed: 30515132
- Shu Y et al. The basal function of teleost prolactin as a key regulator on ion uptake identified with zebrafish knockout models. Sci Rep 6:18597 (2016). PubMed: 26726070