JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB5801

Chemiluminescent HRP Substrate (membrane)

Chemiluminescent HRP Substrate (membrane)

Be the first to review this product! Submit a review

|

(2 Publications)

A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.

Key facts

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Incubate membrane in chemiluminescent substrate for 1 minute then rinse off.</p>" } } }

製品の詳細

A 2 component chemiluminescent substrate for the detection of horseradish peroxidase (HRP) in western blotting.

The Abcam Chemiluminescent HRP Substrate (membrane) is very sensitive and great care must be taken to optimize the individual assay components (antibodies, conjugates, solid phase, etc.) to minimize background reactivity associated with nonspecific immunochemical reactivities

Prepare 1x substrate by mixing 1 ml of the chemiluminescent reagent with 2 ml of the chemiluminescent reaction buffer. Mix well and protect from light. More or less of the 1x substrate can be prepared using the same ratio.

Stability: Stable for a minimum of 18 months when stored at 2-8ºC in the original container and protected from light. The 1x diluted reagent is stable for several hours at room temperature when protected from light.

製品内容

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab5801-1Kit", "assetComponentDetails": [ { "size":"2 x 20 mL", "name":"reaction buffer.", "number":"AB5801-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"luminol reagent.", "number":"AB5801-CMP01", "productcode":"" } ] } } }

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C

製品プロトコール

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 12:2102-2108 PubMed27698699

2016

Decorin reduces hypertrophic scarring through inhibition of the TGF-β1/Smad signaling pathway in a rat osteomyelitis model.

Applications

WB

Species

Unspecified reactive species

Peng Wang,Xiangyan Liu,Peng Xu,Jialiang Lu,Runze Wang,Weidong Mu

PloS one 5:e9861 PubMed20360837

2010

Constitutive expression of pluripotency-associated genes in mesodermal progenitor cells (MPCs).

Applications

Unspecified application

Species

Unspecified reactive species

Simone Pacini,Vittoria Carnicelli,Luisa Trombi,Marina Montali,Rita Fazzi,Edoardo Lazzarini,Stefano Giannotti,Mario Petrini
View all publications

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com