Cell Cytotoxicity Assay Kit (Colorimetric) (ab112118)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
Cell Cytotoxicity Assay Kit (Colorimetric)
Cell Cytotoxicity キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
種交差性
交差種: Mammals, Other species -
製品の概要
Monitoring cell cytotoxicity is one of the most essential tasks for studying cellular functions. There are a variety of parameters that can be used. ab112118 uses a proprietary water-soluble dye that changes its absorption spectra upon cellular reduction. The absorption ratio change is directly proportional to the number of living cells. The characteristics of its high sensitivity, non-radioactivity and no-wash method make ab112118 suitable for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds.
ab112118 does not require pre-mixing of components and has higher sensitivity compared to tetrazolium based colorimetric assays (such as MTT and XTT). It comes with reagents sufficient to run 1000 assays. The kit components are quite stable with minimal cytotoxicity, thus longer incubation times (such as 24-48 hours) are possible if required.
ab112118 is robust and convenient to use. It can be readily adapted for a wide variety of instrument platforms. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
Visit our FAQs page for tips and troubleshooting.
Cytotoxicity assay protocol summary:
- add assay solution to cells and shake for 30 s
- incubate for 1-24 hrs
- analyze with microplate reader -
特記事項
ab112118 should be stored desiccated.
As low as 300 cells can be accurately quantified using ab112118.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 ラベル 1000 tests Assay Solution Component A 1 x 20ml -
研究分野
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画像
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Functional Studies - Cell Cytotoxicity Assay Kit (Colorimetric) (ab112118)
•HeLa: day before experiment cells seeded in 96 wp @ 1e4 cells/well and treated with 1µM, 10 µM and 50 µM Camptothecin (incubated for 24h); on the day of experiment cells incubated with Assay Solution for 2 hours.
•NIH3T3: day before experiment cells seeded in 96 wp @ 1e4 cells/well and treated with 1µM, 10 µM and 50 µM Camptothecin (incubated for 24h); on the day of experiment cells incubated with Assay Solution for 2 hours.
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Functional Studies - Cell Cytotoxicity Assay Kit (Colorimetric) (ab112118)CHO-K1 cell number response was measured with ab112118. CHO-K1 cells at 0 to 10,000 cells/well/100 µL were seeded overnight in a black wall/clear bottom 96-well plate. The cells were incubated with 20 µL/well of Assay Solution for 3 hours at 37 oC. The absorbance intensity was measured at 570 nm and 605 nm. The ratio of OD570/OD605 is proportional to the number of cells as indicated.
データシートおよび資料
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Datasheet download
参考文献 (27)
ab112118 は 27 報の論文で使用されています。
- Brezgin S et al. Hydroxychloroquine Enhances Cytotoxic Properties of Extracellular Vesicles and Extracellular Vesicle-Mimetic Nanovesicles Loaded with Chemotherapeutics. Pharmaceutics 15:N/A (2023). PubMed: 36839856
- Kulkarni B et al. Rhodamine B-Conjugated Fluorescent Block Copolymer Micelles for Efficient Chlorambucil Delivery and Intracellular Imaging. ACS Omega 8:22698-22707 (2023). PubMed: 37396240
- Taglang C et al. Deuterium magnetic resonance spectroscopy enables noninvasive metabolic imaging of tumor burden and response to therapy in low-grade gliomas. Neuro Oncol 24:1101-1112 (2022). PubMed: 35091751
- Khan A et al. Safety, Stability, and Therapeutic Efficacy of Long-Circulating TQ-Incorporated Liposomes: Implication in the Treatment of Lung Cancer. Pharmaceutics 14:N/A (2022). PubMed: 35057049
- Mateo F et al. Modification of BRCA1-associated breast cancer risk by HMMR overexpression. Nat Commun 13:1895 (2022). PubMed: 35393420
