Cell Cycle Assay Kit (Fluorometric - Red) (ab112117)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Fluorescent
- Platform: Flow cytometer
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
Cell Cycle Assay Kit (Fluorometric - Red)
Cell Cycle キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Cell-based (quantitative) -
種交差性
交差種: Mammals, Other species -
製品の概要
ab112117 is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Red CCS1 in fixed cells. The dye passes through a permeabilized membrane and intercalates into cellular DNA. The signal intensity of Nuclear Red CCS1 is directly proportional to DNA content after RNA is degraded by RNase provided in the kit. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be monitored with a flow cytometer at Ex/Em = 490/620 nm (FL2 channel).
Visit our FAQs page for tips and troubleshooting. -
特記事項
Store ab112117 desiccated.
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試験プラットフォーム
Flow cytometer
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 100X RNase A 1 x 250µl Assay Buffer 1 x 50ml Nuclear Red CCS1 1 x 250µl -
研究分野
画像
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Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Red) (ab112117)DNA profile in growing Jurkat cells.
Jurkat cells were dye-loaded with Red Fluorescence Probe and RNase A for 30 minutes. The fluorescence intensity of Red Fluorescence Probe was measured with a flow cytometer, using the FL2 channel.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab112117 は 2 報の論文で使用されています。
- Arabsorkhi Z et al. miR-298 plays a pivotal role in colon cancer invasiveness by targeting PTEN. J Cell Physiol 235:4335-4350 (2020). PubMed: 31621072
- Parada CA et al. Kinome and phosphoproteome of high-grade meningiomas reveal AKAP12 as a central regulator of aggressiveness and its possible role in progression. Sci Rep 8:2098 (2018). Functional Studies . PubMed: 29391485