Cell Cycle Assay Kit (Fluorometric - Green) (ab112116)
Key features and details
- Detection method: Fluorescent
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
Cell Cycle Assay Kit (Fluorometric - Green)
Cell Cycle キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Adherent cells, Suspension cells -
種交差性
交差種: Mammals, Other species -
製品の概要
Cell Cycle Assay Kit (Fluorometric - Green) (ab112116) is designed to monitor cell cycle progression and proliferation by using our proprietary Nuclear Green CCS1 in live cells. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be determined by flow cytometry. Cells stained with Nuclear Green CCS1 can be monitored with a flow cytometer at Ex/Em = 490 nm/520 nm (FL1 channel).
Visit our FAQs page for tips and troubleshooting.
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Assay Buffer 1 x 50ml Nuclear Green CCS1 1 x 250µl -
研究分野
画像
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Flow cytometry - ab112116 Cell Cycle Assay kit (Fluorometric-Green)
Histogram of HeLa cells (black: untreated cells, red : apoptotic cells) stained with 0.1x Nuclear Green CCS1
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Flow Cytometry - Cell Cycle Assay Kit (Fluorometric - Green) (ab112116)DNA profile in growing and camptothecin treated Jurkat cells.
Jurkat cells were treated without (red) or with 20 µM camptothecin (blue) in a 37°C, 5% CO2 incubator for ~8 hours, then dye loaded with 200X Green Fluorescence Probe for 60 minutes. The fluorescence intensity of 200X Green Fluorescence Probe was measured with a flow cytometer using the FL1 channel.
データシートおよび資料
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Datasheet download
参考文献 (15)
ab112116 は 15 報の論文で使用されています。
- Li H et al. Pepsin enhances glycolysis to promote malignant transformation of vocal fold leukoplakia epithelial cells with dysplasia. Eur Arch Otorhinolaryngol 280:1841-1854 (2023). PubMed: 36380093
- Li C et al. SUMO Proteomics Analyses Identify Protein Inhibitor of Activated STAT-Mediated Regulatory Networks Involved in Cell Cycle and Cell Proliferation. J Proteome Res 22:812-825 (2023). PubMed: 36723483
- Zhou X et al. Metabolomics analysis reveals cytotoxic effects of ouabain towards psoriatic keratinocytes via impairment of glutathione metabolism. Mol Genet Genomics 298:567-577 (2023). PubMed: 36856826
- Huang YG et al. CENPF/CDK1 signaling pathway enhances the progression of adrenocortical carcinoma by regulating the G2/M-phase cell cycle. J Transl Med 20:78 (2022). PubMed: 35123514
- Lin H et al. COA3 overexpression promotes non-small cell lung cancer metastasis by reprogramming glucose metabolism. Am J Cancer Res 12:3662-3678 (2022). PubMed: 36119836
