Cathepsin S Activity Assay Kit (Fluorometric) (ab65307)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
製品の概要
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製品名
Cathepsin S Activity Assay Kit (Fluorometric) -
検出方法
Fluorescent -
サンプルの種類
Tissue Extracts, Cell Lysate -
アッセイタイプ
Enzyme activity -
全工程の試験時間
2h 00m -
製品の概要
Cathepsin S Activity Assay Kit (ab65307) is a fluorescence-based assay that uses the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-S will cleave the synthetic substrate VVR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K144 Cathepsin S Activity Fluorometric Assay Kit. K144-100 is the same size as the 100 test size of ab65307.
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Cathepsin Inhibitor 1 x 20µl Cathepsin S Lysis Buffer 1 x 25ml Cathepsin S Assay Buffer 1 x 5ml Cathepsin S Substrate 1 x 200µl -
研究分野
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関連性
The cathepsin family of proteases contains several diverse classes of enzymes. The cysteine protease class comprises cathepsin B, H, K, L, O, and S. The aspartyl protease class contains cathepsin D and E. Cathepsin G belongs to the serine protease class. Cathepsins are involved in various cellular events such as peptide biosynthesis, protein degradation, and apoptosis. Cathepsin S has been shown to be able to function as an elastase over a broad pH range in alveolar macrophages. -
細胞内局在
Lysosome -
別名
- CathepsinS
- CTSS
- MGC3886
画像
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ab65307 Cathepsin S Activity
Cathepsin S Activity measured in tissue lysates (each with protein concentration of 4 mg/mL) after 1 hour of incubation.
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ab65307 Cathepsin S ActivityCathepsin S Activity measured in cell lysates (each with cells concentration of 4e7 cells/mL) after 1 hour of incubation with and without inhibitor (I).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab65307 は 22 報の論文で使用されています。
- Aly R et al. Dysregulation of kidney proteases in the pathogenesis of hypertension following unilateral nephrectomy in juvenile mice. Am J Transl Res 16:544-556 (2024). PubMed: 38463588
- Xie Y et al. Elafin Reverses Intestinal Fibrosis by Inhibiting Cathepsin S-Mediated Protease-Activated Receptor 2. Cell Mol Gastroenterol Hepatol 14:841-876 (2022). PubMed: 35840034
- Karimkhanloo H et al. Circulating cathepsin S improves glycaemic control in mice. J Endocrinol 248:167-179 (2021). PubMed: 33289685
- Fu R et al. Rab27a Contributes to Cathepsin S Secretion in Lacrimal Gland Acinar Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33562815
- Wu J et al. Genome-Wide Screen and Validation of Microglia Pro-Inflammatory Mediators in Stroke. Aging Dis 12:786-800 (2021). PubMed: 34094642