Cathepsin H Activity Assay Kit (Fluorometric) (ab65305)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
製品の概要
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製品名
Cathepsin H Activity Assay Kit (Fluorometric) -
検出方法
Fluorescent -
サンプルの種類
Tissue Extracts, Cell Lysate -
アッセイタイプ
Quantitative -
全工程の試験時間
2h 00m -
製品の概要
Cathepsin H Activity Assay Kit (Fluorometric) (ab65305) is a fluorescence-based assay that utilizes the preferred cathepsin-B substrate Arginine labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-B will cleave the synthetic substrate R-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.The released AFC can easily be quantified using a fluorometer or fluorescence plate reader. The Cathepsin H assay is simple, straightforward, and can be adapted to 96-well plate assays. Assay conditions have been optimized to obtain the maximal activity.
Visit our FAQs page for tips and troubleshooting. -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K145 Cathepsin H Activity Fluorometric Assay Kit. K145-100 is the same size as the 100 test size of ab65305.
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the SDS download section.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests CH Cell Lysis Buffer 1 x 25ml CH Inhibitor (1 mM) 1 x 20µl CH Reaction Buffer 1 x 5ml CH Substrate R-AFC (10 mM) 1 x 200µl -
研究分野
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関連性
The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and O. The aspartyl protease class is composed of cathepsins D and E. Cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in various cellular events such as peptide biosynthesis and protein degradation. The propeptide region of cathepsin H is highly homologous to other cathepsins. This region is thought to play a role in the processing of cysteine proteases. Cathepsin H is thought to be involved in several pathological conditions, including neuro degenerative disorders and cancer. -
細胞内局在
Lysosome -
別名
- ACC 4
- ACC 5
- ACC4
see all
関連製品
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Related Products
画像
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Functional Studies - Cathepsin H Activity Fluorometric Assay Kit (ab65305)Cathepsin H assays were performed using various numbers of Jurkat cells (A) or various amounts of purified human liver cathepsin H (B), as indicated. Results were analyzed using a fluorescence plate reader (Ex/Em = 400/505 nm) as described in the kit instructions.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab65305 は論文での使用が確認できていません。
