Caspase-3 Assay Kit (Colorimetric) (ab39401)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Lysate
製品の概要
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製品名
Caspase-3 Assay Kit (Colorimetric)
Caspase-3 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell Lysate, Tissue Lysate -
アッセイタイプ
Enzyme activity -
全工程の試験時間
2h 00m -
製品の概要
Caspase-3 Assay Kit (Colorimetric) ab39401 provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD.
The Caspase-3 assay protocol is based on the formation of the chromophore p-nitroaniline (p-NA) by cleavage from the labeled substrate DEVD-pNA. The p-NA can be quantified using a spectrophotometer or a microtiter plate reader reading absorbance at 400 or 405 nm.
Comparison of the absorbance of p-NA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-3 activity.
Caspase-3 assay protocol summary:
- add samples to wells
- add reaction buffer and DEVD-p-NA substrate and incubate for 60-120 min at 37ºC
- analyze with microplate reader -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K106 Caspase-3 Colorimetric Assay Kit. K106-100 is the same size as the 100 test size of ab39401.
Due to the nature of the substrate, this assay also detects caspase-7 activity.
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2X Reaction Buffer I 4 x 2ml Lysis Buffer IV 1 x 100ml DEVD-pNA 1 x 500µl Dilution Buffer II 1 x 100ml DTT I 1 x 400µl -
研究分野
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. -
組織特異性
Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. -
配列類似性
Belongs to the peptidase C14A family. -
翻訳後修飾
Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. -
細胞内局在
Cytoplasm. - Information by UniProt
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別名
- A830040C14Rik
- Apopain
- CASP 3
see all
関連製品
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Corresponding Antibody
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Related Products
画像
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Caspase-3 Assay Kit (Colorimetric) (ab39401)Image from Xu G. et al. J Biol Chem. 2012;287(7):4808–4817. doi:10.1074/jbc.M111.275719. Fig. 2G.H9c2 cells were either untreated (control) or treated with 2µg/ml of recombinant lipocalin-2 (Lcn2) for 24h. Cell lysates were assayed for caspase-3 activity (n = 3, *, p<0.05). Image obtained from Xu G et al., JBC, 2012 Feb 11;28(7):4808-17
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Caspase-3 Assay Kit (Colorimetric) (ab39401)Caspase-3 in Jurkat lysates (3.3 x10e6 cells) following 20 hour exposure to 2 uM Camptothecin (ab120115) or 10 ng/mL anti-Fas Ab (MBL).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (287)
ab39401 は 287 報の論文で使用されています。
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- Zhou Y et al. Astragaloside IV ameliorates spinal cord injury through controlling ferroptosis in H2O2-damaged PC12 cells in vitro. Ann Transl Med 10:1176 (2022). PubMed: 36467371
- Lai MC et al. p-Hydroxybenzyl Alcohol Antagonized the ROS-Dependent JNK/Jun/Caspase-3 Pathway to Produce Neuroprotection in a Cellular Model of Parkinson's Disease. Nutrients 14:N/A (2022). PubMed: 36501032
- Delgado M et al. Primary acute lymphoblastic leukemia cells are susceptible to microtubule depolymerization in G1 and M phases through distinct cell death pathways. J Biol Chem 298:101939 (2022). PubMed: 35436470
- Kobutree P et al. Curcumin reduces blood-nerve barrier abnormalities and cytotoxicity to endothelial cells and pericytes induced by cisplatin. Folia Morphol (Warsz) N/A:N/A (2022). PubMed: 35818807