Caspase-2 Assay Kit (Fluorometric) (ab39794)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
製品の概要
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製品名
Caspase-2 Assay Kit (Fluorometric)
Caspase-2 キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Tissue Extracts, Cell Lysate -
アッセイタイプ
Enzyme activity -
全工程の試験時間
2h 00m -
製品の概要
Abcam's Caspase-2 Assay Kit (Fluorometric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on detection of cleavage of substrate VDVAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). VDVAD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified using a fluorometer or a fluorescence microtiter plate reader.
Visit our FAQs page for tips and troubleshooting. -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K116 Caspase-2 Fluorometric Assay Kit. K116-100 is the same size as the 100 test size of ab39794.
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2X Reaction Buffer 4 x 2ml Cell Lysis Buffer 1 x 100ml DTT 1 x 400µl VDVAD-AFC 1 x 500µl -
研究分野
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. -
組織特異性
Expressed at higher levels in the embryonic lung, liver and kidney than in the heart and brain. In adults, higher level expression is seen in the placenta, lung, kidney, and pancreas than in the heart, brain, liver and skeletal muscle. -
配列類似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
翻訳後修飾
The mature protease can process its own propeptide, but not that of other caspases. - Information by UniProt
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別名
- Casp 2L Pro
- CASP-2
- Casp2
see all
関連製品
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Related Products
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab39794 は論文での使用が確認できていません。