Calcium Assay kit (Fluorometric) (ab112115)

The assay buffer in ab112115 is 50 mM Na borate, pH = 8.5.

Component B is a simple sodium borate buffer and therefore is just a dilution buffer.

The optimum pH for this dye is pH8.5. It is quite stable as long as it is protected from the light so you should be able to observe fluorescence after 24 hours without any problems.

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There is always some degree of non specific binding with the fluorophore and plastic of microwell plate. We are not aware if the interaction is chemical however we recommend our customer sot use negative controls in order to deduct the background staining due to non specific binding.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Unfortunately we do not have any data to prove serum interferes with the dye. However, in order to overcome this problem you could add the same amount of serum as sample into the standard.

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In regards to your questions, we would recommend freezing the stock solution instead of the reaction mix, however, there should not be a problem if it is stored at -20C and away from light for up to a week.

I hope this helps. Please do not hesitate to contact us for further assistance.

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Malheureusement, le kit ab112115 ne devrait pas être utilisé dans un milieu acide. Un tampon acide pourrait conduire à des interférences et donc diminuer la fluorescence.

Nous suggérerons aux clients d’utiliser ce kit à un pH optimal de 8,5 avec notre tampon à 50 mM. Vous pouvez commencer avec un pH de 6,5, en utilisant le tampon à une concentration d’environ 10 mM.

Sinon, je vous recommande de regarder sur le site Web Biocompare qui vous permettra de rechercher des produits biologiques et leurs fournisseurs. Le lien est le suivant:
http://www.biocompare.com/

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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The Calcium Quantification Assay kit (Fluorometric), ab112115, is intended for use with solution based samples. For your experiment, it may be better to have a look at the Fluo-8 Medium Removal Calcium Assay kit (ab112128) or the Fluo-8 No Wash Calcium Assay kit (ab112129) to see if you think these would be suitable for you.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to contact us again.

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I have brought your question to the attention of the lab who have informed me that you can wait a few hours with no effect.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for getting back to me.

Yes, the signal would be expected to be enhanced in the presence of zinc.

And in reply to your following question, yes the Fluo-8 (ab112129) and Rhod-4 (ab112157) would not be suitable for your samples. I am sorry for the confusion caused.

If you have any further questions, please do not hesitate to ask.

Thank you for contacting us yesterday in regards to the Calcium Quantification Assay kit (Fluorometric), ab112115.

I now have some further information to share with you in regards to the kit. Unfortunately, I can confirm that the kit is likely to cross-react with zinc (as well as cadmium, mercury, lead and manganese). I am sorry but we do not currently have any kits available from our catalogue where this is not going to be a problem for you (I am confirming this with one final kit and I will get back to you with this information as soon as I have it).

I can however say that the protein in your aqueous solution should not significantly affect the assay.

As discussed over the phone, this kit can measure down to 0.03mM. If you are interested in detecting levels much below this I would suggest having a look at the Fluo-8 No Wash Calcium Assay kit (ab112129). This kit is able to measure much lower calcium concentrations (however, this kit will also pick up zinc).

I hope this information has been of some help. If you have any further questions, please do not hesitate to contact us again.