AB102505

Calcium Assay Kit (Colorimetric)

Name: Calcium Assay Kit (Colorimetric) (ab102505/K380-250) | アブカム
Brand: Abcam Limited
SKU: AB102505
Price: 78000 JPY
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(151 Publications)

Calcium Assay Kit (Colorimetric) ab102505 is a quantitative, mix-and-read assay with just one 10-min incubation step. Readout on any colorimetric (575 nm) plate reader.

- BIOVISION^® assay kit
- Cited in over 100 publications
- Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

別名を表示する

Ca++, Ca2+

6 Images

Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)

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Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)

Ca²⁺ concentration (millimolar) in various biologicals. Shown bars are average of values from dilutions neat to 1 : 8.

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Supplier Data

Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)

Example of standard curve obtained using ab102505

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Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)

Time course of mineralization. Measurement of LDH activity. After a 15-day cultivation period of each of 2 x 10⁶ osteoblasts growing in OSC-supplemented media (OSC+) and control osteoblasts growing in non-OSC-supplemented media (OSC-) the LDH activity in the cell free supernatants was determined as described in Material and Methods. Osteoblasts treated with 1% (v/v) Triton-X100 for 1 hour (c) served as a second control. The experiments were carried out with three primary cultures in triplicate

Görögh, Tibor et al., BMC musculoskeletal disorders-vol. 18,1 500., Fig 3, doi:10.1186/s12891-017-1860-2

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Functional Studies - Calcium Assay Kit (Colorimetric) (AB102505)

Effect of PFOA on calcium concentrations in cultured human osteoblasts after exposure for 3 or 5 weeks. Plots represent mean ± SD.

Koskela, A et al., Scientific reports: vol. 7,1 6841., Fig 4, doi:10.1038/s41598-017-07359-6

Biochemical assay - Calcium Assay Kit (Colorimetric) (AB102505)

Biochemical assay

Lab

Biochemical assay - Calcium Assay Kit (Colorimetric) (AB102505)

Diagram showing the principles of the Calcium assay method.

Other - Calcium Assay Kit (Colorimetric) (AB102505)

Other

Supplier Data

Other - Calcium Assay Kit (Colorimetric) (AB102505)

Representative image of Calcium Assay Kit (Colorimetric) ab102505

Components shown from left to right :

- Calcium Standard

- Calcium Assay Buffer

- Chromogenic Reagent

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

サンプルタイプ

Urine, Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate

検出感度

> 0.1 mM

検出範囲

0.2 - 1 mM

アッセイ時間

20m

アッセイプラットフォーム

Microplate reader

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製品の詳細

Calcium Assay Kit (Colorimetric) ab102505 provides a simple assay to determine calcium concentration within the physiological range of 0.4 – 100 mg/dL (0.2 – 1 mM).

How the assay works
In the calcium assay protocol, a chromogenic complex is formed between calcium ions and 0-cresolphthalein. The complex is measured at OD = 575 nm.

Calcium assay protocol summary

- Add samples and standards to wells
- Add chromogenic reagent, add calcium assay buffer and incubate for 5-10 min at room temp
- Analyze with microplate reader

Related Calcium assay products
Calcium assay kit ab102505 is designed for use with liquid samples such as biological fluids, cell lysates, and tissue extracts.

For the analysis of calcium flux in cell cultures in imaging assays, we supply Fluo-8 Calcium Flux Assay Kits. Calcium Flux Assay Kit (Fluo-8, No Wash) ab112129 is generally preferred as the workflow is simpler. The alternative is Medium removal kit Fluo-8 Calcium Flux Assay Kit ab112128. Note - calcium flus assays are also referred to as calcium influx assays.

For the analysis of calcium flux in cultured cells in a flow cytometer, we supply Calcium Flux Assay Kit (Flow cytometry) (ab233472).

For the imaging of calcium deposits in paraffin sections, we supply Von Kossa (Calcium Stain) Kit ab150687.

The Calcium Assay Buffer component is also available under ab182458.

Getting the best performance from ab102505
This calcium assay detects free calcium ions only.

Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K380 Calcium Colorimetric Assay Kit. K380-250 is the same size as the 250 test size of ab102505.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Calcium often referred to as Ca2+ acts mechanically as an important signaling molecule and structural element in biological systems. It is a small ion with an atomic mass of 40.08 Daltons which allows it to easily move across membranes. You can find calcium expressed in various tissues prominently within bones teeth and intracellular compartments such as the endoplasmic reticulum and mitochondria. As an ion calcium plays an integral role in cellular signaling processes enabling numerous functions including muscle contraction and neurotransmitter release.

Biological function summary

Calcium modulates various cellular activities serving as a messenger in signal transduction pathways. It participates in forming complexes with proteins like calmodulin and troponin which subsequently activate an array of cellular processes. Calcium is also pivotal in maintaining the structural integrity of bones through its deposition as hydroxyapatite. Calcium ions influence cellular excitability and are involved in numerous physiological processes through their interaction with various ion channels enzymes and proteins.

Pathways

Calcium plays critical roles in several including the phosphoinositide signaling pathway and the muscle contraction pathway. In these pathways calcium influx and release modulate activities of proteins like calmodulin and phospholipase C. Calcium’s interactions facilitate the propagation of intracellular signals impacting cell proliferation differentiation and programmed cell death. These pathways highlight calcium’s role in maintaining cellular homeostasis and function.

Calcium imbalance influences conditions such as osteoporosis and cardiac arrhythmias. Dysregulation of calcium homeostasis affects bone density leading to osteoporosis a disorder characterized by increased bone fragility. Furthermore abnormal calcium handling in cardiac myocytes contributes to arrhythmias as calcium ions regulate cardiac muscle excitability and contractility. Proteins like calmodulin and troponin linked with calcium play roles in these conditions highlighting the clinical impact of calcium signaling disturbances.

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製品プロトコール

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ターゲットの情報

See full target information Calcium

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文献 (151)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:22557 PubMed40595199

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A pilot clinical trial to explore the effects of UV exposure on vitamin D synthesis and inflammatory responses in vitamin D-Deficient adults.

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Min-Young Noh,Seong-Il Oh,Young-Eun Kim,Sun Joo Cha,Wonjae Sung,Ki-Wook Oh,Yurim Park,Ji Young Mun,Chang-Seok Ki,Minyeop Nahm,Seung Hyun Kim

PLoS biology 23:e3003164 PubMed40315416

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Cadherin-16 regulates acoustic sensory gating in zebrafish through endocrine signaling.

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Susannah S Schloss,Zackary Q Marshall,Nicholas J Santistevan,Stefani Gjorcheska,Amanda Stenzel,Lindsey Barske,Jessica C Nelson

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Vitamin D receptor signalling regulates the diet-driven metabolic shift during weaning.

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Neha Jawla,Shubhi Khare,Nidhi Yadav,Ranjan Kumar Nanda,G Aneeshkumar Arimbasseri

Cells 14: PubMed40136673

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Blockade of HSP70 Improves Vascular Function in a Mouse Model of Type 2 Diabetes.

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Valentina Ochoa Mendoza,Amanda Almeida de Oliveira,Kenia Pedrosa Nunes

Drug design, development and therapy 19:1489-1502 PubMed40051467

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Mogrol Regulates the Expression of ATPase Na+/K+ Transport Subunit 3, Inhibits Cardiomyocyte Apoptosis, and Plays a Protective Role Against Myocardial Infarction.

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Feng Wang,Jinling Zhou,Weiwei Liu,Wei Wang,Boyan Tian,Jinyu Liu,Han Zhang,Peina He,Xiaoyun Yang,Li Yang,Yueheng Wang

American journal of translational research 17:528-537 PubMed39959245

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The molecular mechanism underlying angiogenesis in a mouse model of chronic kidney disease after ischemic stroke.

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Fang Wang,Liangxiang Lu,Jialun Feng,Jinhua Zheng,Zefeng Wei,Ziqiang Wang

Molecular neurobiology 62:5787-5803 PubMed39630405

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EAAT2 Activation Regulates Glutamate Excitotoxicity and Reduces Impulsivity in a Rodent Model of Parkinson's Disease.

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Sanjay Das,Kyle Mccloskey,Binod Nepal,Sandhya Kortagere

Nature communications 15:10095 PubMed39572569

2024

Calcium nanoparticles target and activate T cells to enhance anti-tumor function.

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Wei Yang,Zhizi Feng,Xinning Lai,Jianwen Li,Zhengwei Cao,Fangchao Jiang,Fanghui Chen,Shuyue Zhan,Feng Kong,Li Yang,Yong Teng,Wendy T Watford,Gang Zhou,Jin Xie

International journal of molecular sciences 25: PubMed39518935

2024

Proteomic Profiling of Endothelial Cell Secretomes After Exposure to Calciprotein Particles Reveals Downregulation of Basement Membrane Assembly and Increased Release of Soluble CD59.

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Alexander Stepanov,Daria Shishkova,Victoria Markova,Yulia Markova,Alexey Frolov,Anastasia Lazebnaya,Karina Oshchepkova,Daria Perepletchikova,Daria Smirnova,Liubov Basovich,Egor Repkin,Anton Kutikhin

View all publications

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Calcium Assay Kit (Colorimetric)