AB272527

Calcium Assay Kit

Name: Calcium Assay Kit (ab272527) | Abcam
Brand: Abcam Limited
SKU: AB272527
Price: 78000 JPY
Availability: InStock

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(2 Publications)

Calcium Assay Kit (ab272527) is a simple, direct and automation-ready procedures for measuring calcium concentration in biological samples.

別名を表示する

Ca++, Ca2+

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Functional Studies - Calcium Assay Kit (AB272527)

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Supplier Data

Functional Studies - Calcium Assay Kit (AB272527)

Calcium Assay Kit Standard Curve.

Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Example Ca²⁺ standard curve.

Key facts

検出方法

Colorimetric

サンプルタイプ

Food samples, Saliva, Urine, Plasma, Other biological fluids, Whole Blood

検出範囲

20 - 5000 µM

アッセイプラットフォーム

Microplate

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製品の詳細

Calcium Assay Kit (ab272527) is a simple, direct and automation-ready procedures for measuring calcium concentration in biological samples. The kit is designed to measure calcium directly in biological samples without any pretreatment. A phenolsulphonephthalein dye in the kit forms a very stable blue colored complex specifically with free calcium. The intensity of the color, measured at 612 nm, is directly proportional to the calcium concentration in the sample. The optimized formulation minimizes any interference by substances such as magnesium, lipid, protein and bilirubin.

Sensitive and accurate. Use as little as 5 μL samples. Linear detection range 0.08 mg/dL (20 μM) to 20 mg/dL (5 mM) Ca²⁺ in 96-well plate assay.

Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 3 min. Can be readily automated as a high-throughput assay for thousands of samples per day.

Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay.

Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc.

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製品内容

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出荷温度及び保存条件

出荷温度

Ambient - Can Ship with Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Calcium ions (Ca²⁺) serve as essential signaling molecules in various cellular processes. These ions have an atomic mass number of 40.08. Calcium ions are highly concentrated in bone tissues teeth and within cells mostly stored in the endoplasmic reticulum and mitochondria. Common indicators of calcium concentration in research include the use of dyes such as Fura-2 and Calbryte 520 AM. These indicators help in visualizing the transient increases in intracellular calcium levels which are important in cellular signaling.

Biological function summary

Calcium ions mediate various physiological functions including muscle contraction neurotransmitter release and hormone secretion. They typically do not act as lone agents; instead they often function as part of protein complexes particularly with calcium-binding proteins like calmodulin and troponin C. Calcium ions also interact with enzymes such as protein kinases and phosphatases regulating metabolic pathways and genetic expression.

Pathways

Calcium ions play pivotal roles in the calcium signaling pathway and the cardiac excitation-contraction coupling pathway. In the calcium signaling pathway calcium ions once released from stores like the endoplasmic reticulum activate proteins such as calmodulin which then modulates various cellular activities. In the cardiac excitation-contraction coupling pathway calcium bows its link to proteins like sarcoplasmic reticulum calcium ATPase essential for heart muscle function.

Calcium ion dysregulation can lead to conditions such as osteoporosis and cardiac arrhythmias. In osteoporosis low calcium levels contribute to decreased bone density affecting bone health and making them prone to fractures. In cardiac arrhythmias abnormal calcium handling can result in irregular heartbeats. Proteins such as calbindin-D28k are closely connected to calcium metabolism disorders highlighting the significance of calcium in maintaining physiological balance.

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製品プロトコール

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ターゲットの情報

See full target information Calcium

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Marine drugs 21: PubMed37999389

2023

Development of Bi- and Tri-Layer Nanofibrous Membranes Based on the Sulfated Polysaccharide Carrageenan for Periodontal Tissue Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanos Kikionis,Konstantina Iliou,Aikaterini G Karra,Georgios Polychronis,Ioannis Choinopoulos,Hermis Iatrou,George Eliades,Efthymia Kitraki,Ioulia Tseti,Spiros Zinelis,Efstathia Ioannou,Vassilios Roussis

Plants (Basel, Switzerland) 12: PubMed37176913

2023

Role of Polyamines in the Response to Salt Stress of Tomato.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Borromeo,Fabio Domenici,Maddalena Del Gallo,Cinzia Forni

View all publications

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Abcam product promise

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Calcium Assay Kit