Annexin V-FITC Apoptosis Staining / Detection Kit

• FuncS

Supplier Data

Functional Studies - Annexin V-FITC Apoptosis Staining / Detection Kit (AB14085)

Annexin V-FITC/ PI staining of AG06173 primary fibroblasts.
10⁵ cells were used for analysis. Resuspended cells were incubated with Annexin V-FITC for 15 min in the dark. Propidium iodide (ab14083) was used as a counterstain to discriminate necrotic/ dead cells from apoptotic cells. Left : negative control - AG6173 untreated cells. Right : positive control - AG6173 cells irradiated at 10 Gy.
Image courtesy of S. Khoronenkova PhD, Gray Institute for Radiation Oncology and Biology, University of Oxford, Oxford, UK.

• FuncS

PubMed

Functional Studies - Annexin V-FITC Apoptosis Staining / Detection Kit (AB14085)

Analysis of apoptosis in prostate cancer cells following treatment with CTA095.

PC3 cells were seeded at 10⁶ cells/ml and incubated overnight and then treated with CTA095 at various concentrations for 24hours. Apoptosis was then analyzed using Annexin-V FITC apoptosis detection kit (ab14085).

This is a modified version of the original image

Guo W et al., PLoS One, 8(8). Fig7b, doi: 10.1371/journal.pone.0070910 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• Flow Cyt

PubMed

Flow Cytometry - Annexin V-FITC Apoptosis Staining / Detection Kit (AB14085)

Flow cytometery analysis of treated HeLa cells for 48 hours.

HeLa cells were harvested with trypsinization together with floating non-viable cells. Cells were washed with PBS and suspended in sodium citrate buffer 20 minutes prior to analysis. HeLa cells were treated with Mitoxantrone (MX) and MX +Imatinib for 48 hours. The samples were then stained with Annexin V-FITC Apoptosis Staining/Detection kit (ab14085). A FACSCalibur flow cytometer was used for cell cycle analysis.

This is a modified version of the original image

Alpay et al., PLos One, 9(19), Fig 5B. Doi:10.1371/journal.pone.0105526 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• ICC/IF

AbReview

Immunocytochemistry/ Immunofluorescence - Annexin V-FITC Apoptosis Staining / Detection Kit (AB14085)

Apoptosis in Mouse Cortical Collecting Duct Cells.

ab14085 was used to determine minor levels of apoptosis (using both the Annexin V-FITC and PI) in mouse cortical collecting duct cellss (mCCDs). mCCD cells were incubated with serum free medium for 48h. The green label on the plasma membrane (Annexin V-FITC) and the absence of nuclear red (PI) staining indicates apoptosis rather than necrosis. Fluorescent microsocpy ws used to analyse the cells.

Image courtesy of an anonymous abreview

• Schematic Diagram

Supplier Data

Schematic Diagram - Annexin V-FITC Apoptosis Staining / Detection Kit (AB14085)

Representative image of Annexin V-FITC Apoptosis Staining / Detection Kit ab14085

Components shown from left to right :

- Propidium Iodide II

- Annexin V-FITC II

- Binding Buffer II

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.