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Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)

Submit a review Q&A (1)References (26)
Impact of different Rep constructs on DNA damage response and apoptosis - ab14150

    Key features and details

    • Assay type: Direct
    • Platform: Flow cytometer, Fluorescence microscope
    • Assay time: 10 min
    • Sample type: Adherent cells, Suspension cells

    製品の概要

    • 製品名

      Annexin V-Cy5 Apoptosis Staining / Detection Kit
      Annexin V/ANXA5 キット 製品一覧

    • サンプルの種類

      Adherent cells, Suspension cells

    • アッセイタイプ

      Direct

    • 全工程の試験時間

      0h 10m

    • 製品の概要

      Annexin V-Cy5 Apoptosis Staining / Detection Kit ab14150 is used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane.


      Fluorescence can be detected at Ex/Em = 649/670 nm.


      Analysis is by flow cytometry or fluorescence microscopy.


      The Annexin V-Cy5 reagent contained in the kit is also available as Annexin V-Cy5 reagent ab14147.

    • 特記事項

      This product is manufactured by BioVision, an Abcam company and was previously called K103 Annexin V-Cy5 Apoptosis Kit. K103-100 is the same size as the 100 test size of ab14150.

      Soon after initiating apoptosis, cells translocate membrane phosphatidylserine molecules from the inner face of the plasma membrane to the cell surface. Phosphatidylserine on the cell surface is detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for phosphatidylserine.

      For more apoptosis assays, review the full set of Annexin V assays, or the apoptosis assay and apoptosis marker guide.

    • 試験プラットフォーム

      Flow cytometer, Fluorescence microscope

    製品の特性

    画像

    • Impact of different Rep constructs on DNA damage response and apoptosis - ab14150
      Impact of different Rep constructs on DNA damage response and apoptosis - ab14150Image from Seyffert M et al., PLoS One 12(1), fig 3d. Doi: 10.1371/journal.pone.0170908. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Vero cells were co-transfected with different Rep-constructs together with a plasmid expressing eGFP to identify successful transfection. After three days, cells were stained using Annexin V-Cy5 Apoptosis staining/detection kit (ab14150) and analyzed by flow cytometry with filters for eGFP and Cy5.  

    参考文献 (26)

    ab14150 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab14150 は 26 報の論文で使用されています。

    • Li P  et al. LIF, a mitogen for choroidal endothelial cells, protects the choriocapillaris: implications for prevention of geographic atrophy. EMBO Mol Med 14:e14511 (2022). PubMed: 34779136
    • Lu J  et al. In vitro assessment of an engineered tBID-based safety switch system in human T lymphocytes. Ann Transl Med 9:1641 (2021). PubMed: 34988150
    • Voigt S  et al. A central role of IKK2 and TPL2 in JNK activation and viral B-cell transformation. Nat Commun 11:685 (2020). PubMed: 32019925
    • Veetil AT  et al. DNA-based fluorescent probes of NOS2 activity in live brains. Proc Natl Acad Sci U S A 117:14694-14702 (2020). PubMed: 32554491
    • Marcar L  et al. Acquired Resistance of EGFR-Mutated Lung Cancer to Tyrosine Kinase Inhibitor Treatment Promotes PARP Inhibitor Sensitivity. Cell Rep 27:3422-3432.e4 (2019). PubMed: 31216465
    View all Publications for this product

    レビューと Q&A

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    Question

    Dear technical team, Our customer is using ab14150 now and would like to know the concentration of the annexin V (component, 1 x 500µl Annexin V-Cy5) Check this please. Thank you.   Best regards,

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    Answer

    Thank you for your enquiry. I can confirm the concentration of annexin V in the ab14150 kit is 30 ug/ml. I hope this will be helpful to you. Should you have any further questions, please do not hesitate to contact us.

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