AB83431

Alpha Ketoglutarate (alpha KG) Assay Kit

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(44 Publications)

Alpha Ketoglutarate (alpha KG) Assay Kit is a mix-and-read assay with one 30-min incubation step. It provides a simple, sensitive and rapid means for quantifying alpha KG.

- Readout on any colorimetric (570 nm) or fluorometric (Ex/Em 535/587 nm) plate reader.

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Functional Studies - Alpha Ketoglutarate (alpha KG) Assay Kit (AB83431)

FuncS

Lab

Functional Studies - Alpha Ketoglutarate (alpha KG) Assay Kit (AB83431)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

FuncS

Lab

Functional Studies - Alpha Ketoglutarate (alpha KG) Assay Kit (AB83431)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Key facts

検出方法

Colorimetric/Fluorometric

サンプルタイプ

Tissue, Suspension cells, Adherent cells

アッセイタイプ

Quantitative

検出感度

= 0.01 nmol/well

検出範囲

0.01 - 10 nmol/well

アッセイ時間

30m

アッセイプラットフォーム

Microplate reader

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製品の詳細

Alpha Ketoglutarate (alpha KG) Assay Kit provides a simple, sensitive and rapid means for quantifying alpha KG.

Alpha ketoglutarate assay principle
In the alpha KG assay protocol, alpha-ketoglutarate is transaminated with the generation of pyruvate, which is used to convert a nearly colorless probe to a colored (λmax = 570 nm) and fluorescent (Ex/Em = 535/587 nm) product.

Alpha Ketoglutarate assay protocol summary
- add reaction mix to standard and sample wells
- incubate for 30 min
- analyze with a microplate reader

Other Notes
This product was previously called K677 Biovision Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit. Biovision was acquired by Abcam in 2021.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-ketoglutarate sometimes called alpha-ketoglutaric acid or A-KG is an important intermediate in several metabolic processes. It plays a mechanical role in the Krebs cycle also known as the citric acid cycle where it functions to transfer energy through oxidative reactions. A-KG is a five-carbon molecule with a mass of approximately 146.11 g/mol and is primarily expressed in the mitochondria of cells particularly within tissues with high metabolic activity such as liver and muscle.

Biological function summary

Alpha-ketoglutarate supports amino acid metabolism and the regulation of gene expression. It acts as an essential nitrogen transporter in biochemical pathways. A-KG serves as a co-substrate for several dioxygenases including prolyl hydroxylases which are enzymes connected to collagen synthesis and the hypoxia-inducible factor pathway. Additionally it is involved in the formation of neurotransmitters and the detoxification of ammonia in animals.

Pathways

Alpha-ketoglutarate is deeply integrated within the tricarboxylic acid cycle and the gamma-aminobutyric acid (GABA) shunt. Within the TCA cycle A-KG forms important energy carriers like NADH and FADH2 impacting energy production. In the GABA shunt it closely associates with glutamate dehydrogenase which transforms glutamate emphasizing its role in nitrogen and energy balance. A-KG interacts with proteins like succinyl-CoA synthetase and is linked with enzymes such as isocitrate dehydrogenase.

Alterations in alpha-ketoglutarate levels have been linked to metabolic disorders like hyperammonemia and certain types of cancer. In hyperammonemia improper detoxification processes through the urea cycle can lead to elevated ammonia levels a problem in which alpha-ketoglutarate plays a detoxifying role. Oncogenic mutations in isocitrate dehydrogenase can lead to abnormal A-KG levels contributing to cancer progression by affecting cellular metabolism and gene expression.

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製品プロトコール

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ターゲットの情報

See full target information 2-Oxoglutaric acid

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文献 (44)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 24:e56009 PubMed37642636

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HDAC6 deacetylates IDH1 to promote the homeostasis of hematopoietic stem and progenitor cells.

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Nature communications 14:4360 PubMed37468519

2023

Nuclear translocation of mitochondrial dehydrogenases as an adaptive cardioprotective mechanism.

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Shubhi Srivastava,Priyanka Gajwani,Jordan Jousma,Hiroe Miyamoto,Youjeong Kwon,Arundhati Jana,Peter T Toth,Gege Yan,Sang-Ging Ong,Jalees Rehman

NAR cancer 5:zcad032 PubMed37388539

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PKM2 dictates the poised chromatin state of promoter to enhance breast cancer progression.

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Madhura R Pandkar,Adarsh Raveendran,Kajal Biswas,Srinivas Abhishek Mutnuru,Jharna Mishra,Atul Samaiya,Tyler Malys,Alexander Y Mitrophanov,Shyam K Sharan,Sanjeev Shukla

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22840 PubMed36943397

2023

P4HA2-mediated HIF-1α stabilization promotes erdafitinib-resistance in FGFR3-alteration bladder cancer.

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Xuexiang Li,Yunxue Li,Bing Liu,Liang Chen,Fang Lyu,Pu Zhang,Qingliu He,Lulin Cheng,Chunyu Liu,Yarong Song,Yifei Xing

International journal of molecular sciences 24: PubMed36834536

2023

Tetrathiomolybdate Decreases the Expression of Alkaline Phosphatase in Dermal Papilla Cells by Increasing Mitochondrial ROS Production.

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Fan Li,Hongli Liu,Xiaojing Wu,Zhicheng Song,Haojia Tang,Maohua Gong,Lei Liu,Fuchang Li

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2207084 PubMed36737855

2023

Extracellularly Detectable Electrochemical Signals of Living Cells Originate from Metabolic Reactions.

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Kyeong-Mo Koo,Chang-Dae Kim,Huijung Kim,Yeon-Woo Cho,Intan Rosalina Suhito,Tae-Hyung Kim

Nature communications 14:288 PubMed36653384

2023

A new AMPK isoform mediates glucose-restriction induced longevity non-cell autonomously by promoting membrane fluidity.

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Jin-Hyuck Jeong,Jun-Seok Han,Youngae Jung,Seung-Min Lee,So-Hyun Park,Mooncheol Park,Min-Gi Shin,Nami Kim,Mi Sun Kang,Seokho Kim,Kwang-Pyo Lee,Ki-Sun Kwon,Chun-A Kim,Yong Ryoul Yang,Geum-Sook Hwang,Eun-Soo Kwon

Cell reports 41:111827 PubMed36516759

2022

EGFR-phosphorylated GDH1 harmonizes with RSK2 to drive CREB activation and tumor metastasis in EGFR-activated lung cancer.

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JiHoon Kang,Jaemoo Chun,Jung Seok Hwang,Chaoyun Pan,Jie Li,Austin C Boese,Isabelle Young,Courteney M Malin,Yibin Kang,Don L Gibbons,Gabriel Sica,Haian Fu,Suresh S Ramalingam,Lingtao Jin,Sumin Kang

Cell reports 41:111744 PubMed36450260

2022

Inhibition of glutaminolysis restores mitochondrial function in senescent stem cells.

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Debanik Choudhury,Na Rong,Izuagie Ikhapoh,Nika Rajabian,Georgios Tseropoulos,Yulun Wu,Pihu Mehrotra,Ramkumar Thiyagarajan,Aref Shahini,Kenneth L Seldeen,Bruce R Troen,Pedro Lei,Stelios T Andreadis

Oxidative medicine and cellular longevity 2022:4938811 PubMed36304962

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MicroRNA 101 Attenuated NSCLC Proliferation through IDH2/HIF Axis Suppression in the Warburg Effect.

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Le Han,Yili Zhang,Bin Zhao,Jianren Yue,Zhenghong Chen,Guangyan Lei,Chen Huang,Wenjuan Chen

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Alpha Ketoglutarate (alpha KG) Assay Kit